Rnasel (NM_011882) Mouse Untagged Clone

CAT#: MC221163

Rnasel (untagged) - Mouse ribonuclease L (2', 5'-oligoisoadenylate synthetase-dependent) (Rnasel), (10ug)



  "NM_011882" in other vectors (4)

CNY 9,500.00


货期*
5周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms E230029I04Rik
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC221163 representing NM_011882
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGACCCCGGATTATAACACACCTCAGGGTGGAACCCCATCAGCGGGAAGTCAGAGGACCGTTGTCG
AAGATGATTCTTCGTTGATCAAAGCTGTTCAGAAGGGAGATGTTGTCAGGGTCCAGCAATTGTTAGAAAA
AGGGGCTGATGCCAATGCCTGTGAAGACACCTGGGGCTGGACACCTTTGCACAACGCAGTGCAAGCTGGC
AGGGTAGACATTGTGAACCTCCTGCTTAGTCATGGTGCTGACCCTCATCGGAGGAAGAAGAATGGGGCCA
CCCCCTTCATCATTGCTGGGATCCAGGGAGATGTGAAACTGCTCGAGATTCTCCTCTCTTGTGGTGCAGA
CGTCAATGAGTGTGACGAGAACGGATTCACGGCTTTCATGGAAGCTGCTGAGCGTGGTAACGCTGAAGCC
TTAAGATTCCTTTTTGCTAAGGGAGCCAATGTGAATTTGCGACGACAGACAACGAAGGACAAAAGGCGAT
TGAAGCAAGGAGGCGCCACAGCTCTCATGAGCGCTGCTGAGAAGGGCCACCTGGAAGTCCTGAGAATTCT
CCTCAATGACATGAAGGCAGAAGTCGATGCTCGGGACAACATGGGCAGAAATGCCCTGATCCGTACTCTG
CTGAACTGGGATTGTGAAAATGTGGAGGAGATTACTTCAATCCTGATTCAGCACGGGGCTGATGTTAACG
TGAGAGGAGAAAGAGGGAAAACACCCCTCATCGCAGCAGTGGAGAGGAAGCACACAGGCTTGGTGCAGAT
GCTCCTGAGTCGGGAAGGCATAAACATAGATGCCAGGGATAACGAGGGCAAGACAGCTCTGCTAATTGCT
GTTGATAAACAACTGAAGGAAATTGTCCAGTTGCTTCTTGAAAAGGGAGCTGATAAGTGTGACGATCTTG
TTTGGATAGCCAGGAGGAATCATGACTATCACCTTGTAAAGCTTCTCCTCCCTTATGTAGCTAATCCTGA
CACCGACCCTCCTGCTGGAGACTGGTCGCCTCACAGTTCACGTTGGGGGACAGCCTTGAAAAGCCTCCAC
AGTATGACTCGACCCATGATTGGCAAACTCAAGATCTTCATTCATGATGACTATAAAATTGCTGGCACTT
CCGAAGGGGCTGTCTACCTAGGGATCTATGACAATCGAGAAGTGGCTGTGAAGGTCTTCCGTGAGAATAG
CCCACGTGGATGTAAGGAAGTCTCTTGTCTGCGGGACTGCGGTGACCACAGTAACTTAGTGGCTTTCTAT
GGAAGAGAGGACGATAAGGGCTGTTTATATGTGTGTGTGTCCCTGTGTGAGTGGACACTGGAAGAGTTCC
TGAGGTTGCCCAGAGAGGAACCTGTGGAGAACGGGGAAGATAAGTTTGCCCACAGCATCCTATTATCTAT
ATTTGAGGGTGTTCAAAAACTACACTTGCATGGATATTCCCATCAGGACCTGCAACCACAAAACATCTTA
ATAGATTCCAAGAAAGCTGTCCGGCTGGCAGATTTTGATCAGAGCATCCGATGGATGGGAGAGTCACAGA
TGGTCAGGAGAGACTTGGAGGATCTTGGACGGCTGGTTCTCTACGTGGTAATGAAAGGTGAGATCCCCTT
TGAGACACTAAAGACTCAGAATGATGAAGTGCTGCTTACAATGTCTCCAGATGAGGAGACTAAGGACCTC
ATTCATTGCCTGTTTTCTCCTGGAGAAAATGTCAAGAACTGCCTGGTAGACCTGCTTGGCCATCCTTTCT
TTTGGACTTGGGAGAACCGCTATAGAACACTCCGGAATGTGGGAAATGAATCTGACATCAAAGTACGGAA
ATGTAAAAGTGATCTTCTCAGACTACTGCAGCATCAGACACTTGAGCCTCCCAGAAGCTTTGACCAGTGG
ACATCTAAGATCGACAAAAATGTTATGGATGAAATGAATCATTTCTACGAAAAGAGAAAAAAAAACCCTT
ATCAGGATACTGTAGGTGATCTGCTGAAGTTTATTCGGAATATAGGCGAACACATCAATGAGGAAAAAAA
GCGGGGAATGAAGGAGATACTTGGAGATCCTTCTCGTTATTTTCAGGAGACATTTCCAGATCTTGTAATC
TATATCTACAAGAAACTAAAGGAAACAGAATACAGAAAACACTTCCCACAGCCTCCACCACGCCTCAGCG
TTCCAGAGGCAGTGGGACCAGGGGGCATACAGAGCTGA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_011882
Insert Size 2208 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_011882.2, NP_036012.1
RefSeq Size 4758 bp
RefSeq ORF 2208 bp
Locus ID 24014
UniProt ID Q05921
Gene Summary Endoribonuclease that functions in the interferon (IFN) antiviral response. In INF treated and virus infected cells, RNASEL probably mediates its antiviral effects through a combination of direct cleavage of single-stranded viral RNAs, inhibition of protein synthesis through the degradation of rRNA, induction of apoptosis, and induction of other antiviral genes. RNASEL mediated apoptosis is the result of a JNK-dependent stress-response pathway leading to cytochrome c release from mitochondria and caspase-dependent apoptosis. Therefore, activation of RNASEL could lead to elimination of virus infected cells under some circumstances. In the crosstalk between autophagy and apoptosis proposed to induce autophagy as an early stress response to small double-stranded RNA and at later stages of prolonged stress to activate caspase-dependent proteolytic cleavage of BECN1 to terminate autophagy and promote apoptosis. Might play a central role in the regulation of mRNA turnover (By similarity). Cleaves 3' of UpNp dimers, with preference for UU and UA sequences, to sets of discrete products ranging from between 4 and 22 nucleotides in length (By similarity).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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