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Rnasel (NM_011882) Mouse Tagged Lenti ORF Clone
CAT＃: MR219460L3
- LentiORF®
Lenti ORF clone of Rnasel (Myc-DDK-tagged) - Mouse ribonuclease L (2', 5'-oligoisoadenylate synthetase-dependent) (Rnasel)
"NM_011882" in other vectors (4)
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CNY 7,700.00
Product images
CNY 600.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | E230029I04Rik |
| Vector | pLenti-C-Myc-DDK-P2A-Puro |
| E. coli Selection | Chloramphenicol (34 ug/mL) |
| Mammalian Cell Selection | Puromycin |
| Sequence Data |
The ORF insert of this clone is exactly the same as(MR219460).
|
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_011882 |
| ORF Size | 2205 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_011882.2, NP_036012.1 |
| RefSeq Size | 4758 bp |
| RefSeq ORF | 2208 bp |
| Locus ID | 24014 |
| UniProt ID | Q05921 |
| Gene Summary | Endoribonuclease that functions in the interferon (IFN) antiviral response. In INF treated and virus infected cells, RNASEL probably mediates its antiviral effects through a combination of direct cleavage of single-stranded viral RNAs, inhibition of protein synthesis through the degradation of rRNA, induction of apoptosis, and induction of other antiviral genes. RNASEL mediated apoptosis is the result of a JNK-dependent stress-response pathway leading to cytochrome c release from mitochondria and caspase-dependent apoptosis. Therefore, activation of RNASEL could lead to elimination of virus infected cells under some circumstances. In the crosstalk between autophagy and apoptosis proposed to induce autophagy as an early stress response to small double-stranded RNA and at later stages of prolonged stress to activate caspase-dependent proteolytic cleavage of BECN1 to terminate autophagy and promote apoptosis. Might play a central role in the regulation of mRNA turnover (By similarity). Cleaves 3' of UpNp dimers, with preference for UU and UA sequences, to sets of discrete products ranging from between 4 and 22 nucleotides in length (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC221163 | Rnasel (untagged) - Mouse ribonuclease L (2', 5'-oligoisoadenylate synthetase-dependent) (Rnasel), (10ug) |
CNY 9,500.00 |
|
| MG219460 | Rnasel (tGFP-tagged) - Mouse ribonuclease L (2' 5'-oligoisoadenylate synthetase-dependent) (Rnasel), (10ug) |
CNY 6,370.00 |
|
| MR219460 | Rnasel (Myc-DDK-tagged) - Mouse ribonuclease L (2', 5'-oligoisoadenylate synthetase-dependent) (Rnasel) |
CNY 5,384.00 |
|
| MR219460L4 | Lenti ORF clone of Rnasel (mGFP-tagged) - Mouse ribonuclease L (2', 5'-oligoisoadenylate synthetase-dependent) (Rnasel) |
CNY 7,700.00 |
