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P3h3 (NM_013534) Mouse Untagged Clone
CAT＃: MC221153
Leprel2 (untagged) - Mouse leprecan-like 2 (Leprel2), (10ug)
CNY 9,500.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | BC016431; Grcb; Leprel2 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC221153 representing NM_013534
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTCCGGCTCCTGCGGTTGTTGCTTTTGCTGCTGCTGCCTCCCCCGGGGTCCCCCGAGCCTCCCGAGC CCCCGGGCCTGGCTCAGTTGTCACCGGGGTCGCCTCCCCAGGCCCCCGACTTGCTCTACGCGGACGGGCT GCGAGCCTACTCGGCCGGGGCTTGGGCGCCCGCAGTGGCGCTGCTGCGAGAGGCGCTGCGGAGCCGGGCG GCGCTGGGCCGCGCGCGCCAGGAGTGCGGGGCGAGCTGCGCGGCCGAGCCGGGCGCCGCGCTCCCCTCCC AGCTCCTCGGAGCCCCACATCCTGTCTCCGGGCCCGGGGTCTGGGAACCGCTGCTCCTGCGCGCCACGCT CCGCCGCGCCGAGTGCCTGACCCAGTGCGCGGTGCGGAGGCTGGGCCCGGGGGGCGCGGCGCGGCTCCGC GTGGGGAGCGCGCTGCGGGATGCCTTCCGCCGGCGGGAGCCTTACAACTACCTGCAGAGGGCCTACTACC AGTTGAAGAAGCTGGACCTGGCAGCTTCTGCAGCACACACCTTCTTTGTGGCAAACCCAACACACCTGCA GATGCGGGAAGACATGGCTAAGTACAGAAGGATGTCTGCAATCCGACCCCAGAGCTTCCGGGACCTGGTG ACGCCCCTATACTGGGCAGCTTATGACACTGGCCTGGAGCTTCTGGAGCAACGGGAGGCAGCACTGGCTC TACCCCAACTAGAGGAGGCCCTGCAGGGGAGCCTGGCCCACATGGAGAGCTGCCGTGCTGCCTGTGAGGG GCCTGAGGAGCACCAAGGGGCTGAAGAAGAGGGAGAAGGGAGCCAGGGAGGCCTGTATGAAGCCATTGCA GGACACTGGATACGGGTTCTGCAGTGCAGGCAGCACTGTGTGGCAGACACGGCCACCCGTCCTGGTCGCA GCTTCCCTGTCCAGGACTTCCTCCTCAGCCAGCTGAGACGGCTGCACGAGGCGTATGCTCAGGTGGGGAA CATGTCTCAGGCCATGGAAAATGTCCTGAGTGTCCTGCTCTTCTACCCAGAGGATGAGGCTGCCAAAAAG GCTCTGAACCAGTACCAAACTCAGTTGGGAGAGCCAAGACCTGACCTGGGACCACGAGAGGACATCCAGC GGTTCATCCTTCGCTCCCTCGGAGAGAAGAGACAGTTATATTACGCCATGGAGCATCTGGGCACTAGCTT CAAGGACCCGGATTCTTGGACCCCGGAGGCTCTCATCCCTAAGGCACTGAGAGAGAGGCTCAGAGAGGAT CAAGAGAAGAAGCCCTGGGACCATCAGCCTCCACAGCAGAAGCCCTTGGCTCACTGGAAGGATGCCCTTC TGATGGAGGGTGTGACCCTCACGCAGGACGCCCAGCAGCTGAATGGGTCGGAGCGAGCTGTCTTAGATGG GCTGCTAACTTCAGCCGAGTGTGGGGTTCTGCTGCAGCTGGCCAAGGATGCAGCACAGGCTGGAGCCAGG TCAGGCTACCGTGGCCGCCGCTCTCCTCATAGTCCTCATGAACGCTTTGAGGGGCTCACGGTGCTCAAGG CTGCTCAGCTGGCCCGGGCAGGGACTGTGGGCAGGCCAGGCGCTAAGCTGCTTCTGGAGGTAAGTGAGCG AGTGCGGACTTTGACCCAGGCCTACTTCTCCCCGGAACGGCCACTGCATCTTTCCTTCACCCACCTGGTG TGCCGAAGTGCCATAGAAGGTGAACAAGAGCAGCGTATGGACCTGAGTCACCCGGTTCATGCAGACAACT GTGTCCTGGACCCCGACACTGGCGAATGCTGGCGGGAGCCCCCAGCCTACACCTATCGAGACTATAGTGG ACTCCTCTACCTCAATGATGACTTCAAGGGAGGCGACCTGTTCTTCACGCAGCCCAACGCCCTCACTGTC ACGGCTCAGGTCCGTCCTCGGTGTGGGCGCCTTGTGGCCTTCAGCTCTGGTGGTGAGAATCCCCATGGTG TATGGGCTGTGACTCGGGGACGGCGCTGTGCCCTAGCACTGTGGCACACGTGGGCACCTGAGCACAGTGA ACAGGAGTGGACAGAAGCCAAAGAGCTGCTGCAGGAGGAAGAGGAGGAAGAAGAGGAGGAAGACATTCTC AGCAGAGACCCTTCCCCAGAACCCCCAAGTCACAAGCTTCAGCGAGTCCAGGAGAAAGCTGGGAAGCCCC GCCGGGTCCGGGTCCGAGAGGAACTGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_013534 |
| Insert Size | 2199 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_013534.4, NP_038562.2 |
| RefSeq Size | 2779 bp |
| RefSeq ORF | 2199 bp |
| Locus ID | 14789 |
| UniProt ID | Q8CG70 |
| Gene Summary | Part of a complex composed of PLOD1, P3H3 and P3H4 that catalyzes hydroxylation of lysine residues in collagen alpha chains and is required for normal assembly and cross-linkling of collagen fibrils (PubMed:27119146). Required for normal hydroxylation of lysine residues in type I collagen chains in skin, bone, tendon, aorta and cornea (PubMed:28115524). Required for normal skin stability via its role in hydroxylation of lysine residues in collagen alpha chains and in collagen fibril assembly (PubMed:27119146, PubMed:28115524). Apparently not required for normal prolyl 3-hydroxylation on collagen chains, possibly because it functions redundantly with other prolyl 3-hydroxylases (PubMed:28115524).[UniProtKB/Swiss-Prot Function] |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG224833 | Leprel2 (tGFP-tagged) - Mouse leprecan-like 2 (Leprel2), (10ug) |
CNY 8,080.00 |
|
| MR224833 | Leprel2 (Myc-DDK-tagged) - Mouse leprecan-like 2 (Leprel2) |
CNY 7,320.00 |
|
| MR224833L3 | Lenti ORF clone of Leprel2 (Myc-DDK-tagged) - Mouse leprecan-like 2 (Leprel2) |
CNY 9,220.00 |
|
| MR224833L4 | Lenti ORF clone of Leprel2 (mGFP-tagged) - Mouse leprecan-like 2 (Leprel2) |
CNY 9,220.00 |
