P3h3 (NM_013534) Mouse Tagged ORF Clone
CAT#: MG224833
- TrueORF®
Leprel2 (tGFP-tagged) - Mouse leprecan-like 2 (Leprel2), (10ug)
ORF Plasmid: DDK
"NM_013534" in other vectors (4)
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CNY 8,080.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | BC016431; Grcb; Leprel2 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG224833 representing NM_013534
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTCCGGCTCCTGCGGTTGTTGCTTTTGCTGCTGCTGCCTCCCCCGGGGTCCCCCGAGCCTCCCGAGC CCCCGGGCCTGGCTCAGTTGTCACCGGGGTCGCCTCCCCAGGCCCCCGACTTGCTCTACGCGGACGGGCT GCGAGCCTACTCGGCCGGGGCTTGGGCGCCCGCAGTGGCGCTGCTGCGAGAGGCGCTGCGGAGCCGGGCG GCGCTGGGCCGCGCGCGCCAGGAGTGCGGGGCGAGCTGCGCGGCCGAGCCGGGCGCCGCGCTCCCCTCCC AGCTCCTCGGAGCCCCACATCCTGTCTCCGGGCCCGGGGTCTGGGAACCGCTGCTCCTGCGCGCCACGCT CCGCCGCGCCGAGTGCCTGACCCAGTGCGCGGTGCGGAGGCTGGGCCCGGGGGGCGCGGCGCGGCTCCGC GTGGGGAGCGCGCTGCGGGATGCCTTCCGCCGGCGGGAGCCTTACAACTACCTGCAGAGGGCCTACTACC AGTTGAAGAAGCTGGACCTGGCAGCTTCTGCAGCACACACCTTCTTTGTGGCAAACCCAACACACCTGCA GATGCGGGAAGACATGGCTAAGTACAGAAGGATGTCTGCAATCCGACCCCAGAGCTTCCGGGACCTGGTG ACGCCCCTATACTGGGCAGCTTATGACACTGGCCTGGAGCTTCTGGAGCAACGGGAGGCAGCACTGGCTC TACCCCAACTAGAGGAGGCCCTGCAGGGGAGCCTGGCCCACATGGAGAGCTGCCGTGCTGCCTGTGAGGG GCCTGAGGAGCACCAAGGGGCTGAAGAAGAGGGAGAAGGGAGCCAGGGAGGCCTGTATGAAGCCATTGCA GGACACTGGATACGGGTTCTGCAGTGCAGGCAGCACTGTGTGGCAGACACGGCCACCCGTCCTGGTCGCA GCTTCCCTGTCCAGGACTTCCTCCTCAGCCAGCTGAGACGGCTGCACGAGGCGTATGCTCAGGTGGGGAA CATGTCTCAGGCCATGGAAAATGTCCTGAGTGTCCTGCTCTTCTACCCAGAGGATGAGGCTGCCAAAAAG GCTCTGAACCAGTACCAAACTCAGTTGGGAGAGCCAAGACCTGACCTGGGACCACGAGAGGACATCCAGC GGTTCATCCTTCGCTCCCTCGGAGAGAAGAGACAGTTATATTACGCCATGGAGCATCTGGGCACTAGCTT CAAGGACCCGGATTCTTGGACCCCGGAGGCTCTCATCCCTAAGGCACTGAGAGAGAGGCTCAGAGAGGAT CAAGAGAAGAAGCCCTGGGACCATCAGCCTCCACAGCAGAAGCCCTTGGCTCACTGGAAGGATGCCCTTC TGATGGAGGGTGTGACCCTCACGCAGGACGCCCAGCAGCTGAATGGGTCGGAGCGAGCTGTCTTAGATGG GCTGCTAACTTCAGCCGAGTGTGGGGTTCTGCTGCAGCTGGCCAAGGATGCAGCACAGGCTGGAGCCAGG TCAGGCTACCGTGGCCGCCGCTCTCCTCATAGTCCTCATGAACGCTTTGAGGGGCTCACGGTGCTCAAGG CTGCTCAGCTGGCCCGGGCAGGGACTGTGGGCAGGCCAGGCGCTAAGCTGCTTCTGGAGGTAAGTGAGCG AGTGCGGACTTTGACCCAGGCCTACTTCTCCCCGGAACGGCCACTGCATCTTTCCTTCACCCACCTGGTG TGCCGAAGTGCCATAGAAGGTGAACAAGAGCAGCGTATGGACCTGAGTCACCCGGTTCATGCAGACAACT GTGTCCTGGACCCCGACACTGGCGAATGCTGGCGGGAGCCCCCAGCCTACACCTATCGAGACTATAGTGG ACTCCTCTACCTCAATGATGACTTCAAGGGAGGCGACCTGTTCTTCACGCAGCCCAACGCCCTCACTGTC ACGGCTCAGGTCCGTCCTCGGTGTGGGCGCCTTGTGGCCTTCAGCTCTGGTGGTGAGAATCCCCATGGTG TATGGGCTGTGACTCGGGGACGGCGCTGTGCCCTAGCACTGTGGCACACGTGGGCACCTGAGCACAGTGA ACAGGAGTGGACAGAAGCCAAAGAGCTGCTGCAGGAGGAAGAGGAGGAAGAAGAGGAGGAAGACATTCTC AGCAGAGACCCTTCCCCAGAACCCCCAAGTCACAAGCTTCAGCGAGTCCAGGAGAAAGCTGGGAAGCCCC GCCGGGTCCGGGTCCGAGAGGAACTG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG224833 representing NM_013534
Red=Cloning site Green=Tags(s) MLRLLRLLLLLLLPPPGSPEPPEPPGLAQLSPGSPPQAPDLLYADGLRAYSAGAWAPAVALLREALRSRA ALGRARQECGASCAAEPGAALPSQLLGAPHPVSGPGVWEPLLLRATLRRAECLTQCAVRRLGPGGAARLR VGSALRDAFRRREPYNYLQRAYYQLKKLDLAASAAHTFFVANPTHLQMREDMAKYRRMSAIRPQSFRDLV TPLYWAAYDTGLELLEQREAALALPQLEEALQGSLAHMESCRAACEGPEEHQGAEEEGEGSQGGLYEAIA GHWIRVLQCRQHCVADTATRPGRSFPVQDFLLSQLRRLHEAYAQVGNMSQAMENVLSVLLFYPEDEAAKK ALNQYQTQLGEPRPDLGPREDIQRFILRSLGEKRQLYYAMEHLGTSFKDPDSWTPEALIPKALRERLRED QEKKPWDHQPPQQKPLAHWKDALLMEGVTLTQDAQQLNGSERAVLDGLLTSAECGVLLQLAKDAAQAGAR SGYRGRRSPHSPHERFEGLTVLKAAQLARAGTVGRPGAKLLLEVSERVRTLTQAYFSPERPLHLSFTHLV CRSAIEGEQEQRMDLSHPVHADNCVLDPDTGECWREPPAYTYRDYSGLLYLNDDFKGGDLFFTQPNALTV TAQVRPRCGRLVAFSSGGENPHGVWAVTRGRRCALALWHTWAPEHSEQEWTEAKELLQEEEEEEEEEDIL SRDPSPEPPSHKLQRVQEKAGKPRRVRVREEL TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_013534 |
ORF Size | 2196 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_013534.5 |
RefSeq Size | 2779 bp |
RefSeq ORF | 2199 bp |
Locus ID | 14789 |
UniProt ID | Q8CG70 |
Gene Summary | Part of a complex composed of PLOD1, P3H3 and P3H4 that catalyzes hydroxylation of lysine residues in collagen alpha chains and is required for normal assembly and cross-linkling of collagen fibrils (PubMed:27119146). Required for normal hydroxylation of lysine residues in type I collagen chains in skin, bone, tendon, aorta and cornea (PubMed:28115524). Required for normal skin stability via its role in hydroxylation of lysine residues in collagen alpha chains and in collagen fibril assembly (PubMed:27119146, PubMed:28115524). Apparently not required for normal prolyl 3-hydroxylation on collagen chains, possibly because it functions redundantly with other prolyl 3-hydroxylases (PubMed:28115524).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC221153 | Leprel2 (untagged) - Mouse leprecan-like 2 (Leprel2), (10ug) |
CNY 9,500.00 |
|
MR224833 | Leprel2 (Myc-DDK-tagged) - Mouse leprecan-like 2 (Leprel2) |
CNY 7,320.00 |
|
MR224833L3 | Lenti ORF clone of Leprel2 (Myc-DDK-tagged) - Mouse leprecan-like 2 (Leprel2) |
CNY 9,220.00 |
|
MR224833L4 | Lenti ORF clone of Leprel2 (mGFP-tagged) - Mouse leprecan-like 2 (Leprel2) |
CNY 9,220.00 |