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Krit1 (NM_001170552) Mouse Untagged Clone
CAT＃: MC220603
Krit1 (untagged) - Mouse KRIT1, ankyrin repeat containing (Krit1), transcript variant 2, (10ug)
CNY 5,112.00
CNY 9,030.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | 2010007K12Rik; A630036P20Rik; AA432855; AI450393; AI643869; BB155247; BB235701; Ccm1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC220603 representing NM_001170552
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGAAATCCAGAAAACATCGAAGATGCTTACGTTGCAGTTATTCGTCCAAAGAACACTGCTAGTCTCA ACTCCCGGGAGTATAGAGCTAAGTCCTATGAAATTTTATTGCATGAAGTTCCCATTGAAGGACAGAAAAA AAAGCGAAAGAAAGTTTTGCTGGAAACTAAACTTCAAAGCAACAGTGAAATAGCACAAGGCATATTGGAC TATGTAGTAGAAACTACCAAACCAATTTCTCCTGCAAACCAGGGGATTAAAGGGAAACGAGTGGTGCTGA TGAGGAAGTTTCCTCTGGACGGAGAGAAGACAGGCAGAGAAGCAGCACTGTTTATCGTGCCATCAGTTGT CAAAGATAATACTAAATATGCATATACTCCTGGATGCCCAATTTTTTACTGCTTACAAGATATTATGAGA GTTTGTAGTGAATCCAGTACTCACTTTGCAACACTTACAGCAAGGATGTTAATAGCCTTGGATAAGTGGT TAGATGAACGTCATGCGCAGTCTCACTTTATTCCAGCTTTATTCCGACCTTCTCCCCTTGAACGGATAAA GACAAATGTCATAAACCCTGCGTATGCTGCTGAATTAGGCCAGGTAGACAATTCACTACATATGGGCTAT AGTGCACTAGAAATAAAGAGTAAAATGCTAGCCCTAGAGAAAGCAGACACCTGCATTTACAACCCTTTGT TTGGATCAGATCTTCAGTATACAAATCGGGTAGATAAAGTGGTAATAAATCCATACTTTGGTCTCGGAGC TCCAGACTACTCAAAAATCCAAATTCCCAAACAGGAAAAATGGCAGCGAAGCATGAGCAGCGTTGTGGAA GACAAAGAACGACAGTGGGTTGATGACTTTCCTTTACATCGAAATGCCTGTGAAGGAGATTCAGAATTAC TGAGCCATCTTCTCGATAAAGGACTTTCAGTCAACCAACTAGATAATGACCACTGGGCACCCATTCATTA TGCATGCTGGTATGGAAAAGTTGAGGCCACTCGCATATTATTAGAGAAAGGAAAGTGCAATCCAAACCTT TTAAATGGGCAGCTCAGCTCACCGCTTCACTTTGCTGCTGGAGGCGGCCATGCTGAAATAGTGCAGATCC TCCTGACTCACCCAGACATTGACAGGCACATAACAGATCAACAAGGAAGATCCCCATTAAATGTTTGTGA AGAAAACAAACAAAATAACTGGGAAGAAGCTGCAAAATTGTTGAAAGACGCCATTAACAAGCCATATGAA AAAGTTCGAATCTATAGAATGGATGGATCATACCGTTCTGTTGAACTAAAGCATGGCAATAATACCACAG CACAGCAGATAATGGAGGGAATGCGGCTCTCTCAGGAAACTCAGCGATATTTCACTATTTGGATCTGTTC AGAAAATCTTAGTCTTCAGTTCAAGCCTTATCATAAACCCTTGCAACAAGTTCATGACTGGCCAGAAATA CTTGCTGAATTGACTAATTTGGATCCACAAAGAGAAACACCACAGCTTTTCCTAAGAAGAGATGTGGGAC TTCCTTTAGAAGTTGAGAAAAAGATTGAAGACCCACTAGCTATTCTTATTCTCTTTGATGAAGCCAGATA TAATTTACTGAAGGGCTTTTATACAGCTCCTGATGCTAAACTGATAACACTGGCAAGTCTACTGTTACAA ATAGTTTATGGGAATTATGAGAGTAAAAAGCACAAACAAGGTTTCTTAAATGAAGAAACTCTGAAATCCA TCGTACCTATTACTAAACTGAAAAGTAAGGCGCCTCACTGGATAAACCGAATACTCCATGAGTACAAGAA TCTGAGTCTGAGTGAGGGCGTCAGTAAGGAAATGCACCACCTGCAGCGCATGTTCCTACAGAACTGCTGG GAGATCCCTACGTACGGAGCCGCCTTCTTCACAGGACAGATATTTACAAAGGCAAGCCCAAGCAATCATA AAGTCATCCCTGTGTATGTAGGAGTGAATATAAAAGGACTTCATCTCCTGAACATGGAAACTAAGGCTGG CCTTGTGGTAAAGCTGCTAATGAAGTTAAATGGACAACTAATGCCCTCTGAAAGAAATTCATGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_001170552 |
| Insert Size | 2094 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001170552.1, NP_001164023.1 |
| RefSeq Size | 6034 bp |
| RefSeq ORF | 2094 bp |
| Locus ID | 79264 |
| UniProt ID | Q6S5J6 |
| Gene Summary | Component of the CCM signaling pathway which is a crucial regulator of heart and vessel formation and integrity. Negative regulator of angiogenesis. Inhibits endothelial proliferation, apoptosis, migration, lumen formation and sprouting angiogenesis in primary endothelial cells. Promotes AKT phosphorylation in a NOTCH-dependent and independent manner, and inhibits ERK1/2 phosphorylation indirectly through activation of the DELTA-NOTCH cascade. Acts in concert with CDH5 to establish and maintain correct endothelial cell polarity and vascular lumen and these effects are mediated by recruitment and activation of the Par polarity complex and RAP1B. Required for the localization of phosphorylated PRKCZ, PARD3, TIAM1 and RAP1B to the cell junction, and cell junction stabilization. Plays a role in integrin signaling via its interaction with ITGB1BP1; this prevents the interaction between ITGB1 and ITGB1BP1. Microtubule-associated protein that binds to phosphatidylinositol 4,5-bisphosphate (PIP2)-containing membranes in a GTP-bound RAP1-dependent manner (By similarity). Plays an important role in the maintenance of the intracellular reactive oxygen species (ROS) homeostasis to prevent oxidative cellular damage. Regulates the homeostasis of intracellular ROS through an antioxidant pathway involving FOXO1 and SOD2. Facilitates the down-regulation of cyclin-D1 (CCND1) levels required for cell transition from proliferative growth to quiescence by preventing the accumulation of intracellular ROS through the modulation of FOXO1 and SOD2 levels.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) differs in the 5' UTR and lacks an alternate in-frame exon, compared to variant 1, resulting in a shorter protein (isoform 2), compared to isoform 1. Isoform 2 is also known as Krit1B. |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG226178 | Krit1 (tGFP-tagged) - Mouse KRIT1 ankyrin repeat containing (Krit1) transcript variant 2, (10ug) |
CNY 7,600.00 |
|
| MR226178 | Krit1 (Myc-DDK-tagged) - Mouse KRIT1, ankyrin repeat containing (Krit1), transcript variant 2 |
CNY 6,940.00 |
|
| MR226178L3 | Lenti ORF clone of Krit1 (Myc-DDK-tagged) - Mouse KRIT1, ankyrin repeat containing (Krit1), transcript variant 2 |
CNY 8,840.00 |
|
| MR226178L4 | Lenti ORF clone of Krit1 (mGFP-tagged) - Mouse KRIT1, ankyrin repeat containing (Krit1), transcript variant 2 |
CNY 8,840.00 |
