United States
Germany
Japan
United Kingdom
China
Plcz1 (NM_054066) Mouse Untagged Clone
CAT＃: MC220110
Plcz1 (untagged) - Mouse phospholipase C, zeta 1 (Plcz1), (10ug)
CNY 8,360.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | 1700041H07Rik; PLCzeta |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC220110 representing NM_054066
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAAAGCCAACTTCATGAGCTCGCAGAAGCAAGATGGTTTTTGTCAAAGGTTCAGGATGATTTTAGAG GTGGAAAAATCAACGTTGAAATTACTCACAAACTGCTTGAGAAACTTGATTTCCCATGCCACTTTGCTCA TGTGAAACATATTTTTAAGGAAAATGACAGACAGAACCAAGGAAGAATCACCATTGAAGAGTTTAGAGCC ATTTACCGGTGTATTGTACATAGAGAAGAAATCACGGAGATTTTCAACACGTATACTGAAAATAGGAAAA TTCTTTCTGAGAACAGTCTGATTGAGTTTCTAACCCAAGAGCAGTATGAAATGGAGATCGATCACTCTGA TTCAGTAGAGATCATCAATAAGTATGAGCCCATTGAAGAAGTAAAGGGTGAGCGACAGATGTCAATTGAA GGTTTCGCAAGATACATGTTTTCATCAGAATGTCTACTGTTTAAAGAGAACTGTAAAACCGTGTACCAAG ATATGAATCATCCATTAAGTGATTATTTTATTTCATCATCTCACAACACATATTTGATATCCGATCAAAT ATTGGGACCGAGTGACATTTGGGGATATGTAAGTGCTCTTGTGAAAGGCTGCCGCTGTCTGGAAATTGAC TGCTGGGATGGATCCCAAAATGAACCCATTGTGTACCATGGTTACACATTCACCAGCAAGCTTCTCTTCA AAACTGTGGTCCAAGCAATAAACAAGTATGCCTTTGTGACATCTGATTACCCAGTAGTGCTGTCCTTAGA AAATCACTGCTCCCCTGGTCAGCAGGAAGTGATGGCTAGCATTCTGCAGAGCACCTTTGGAGACTTCCTG CTTTCGGACATGCTTGAGGAGTTTCCAGATACACTACCGTCTCCAGAGGCACTAAAATTCAAAATATTAG TGAAAAACAGGAAAGTGGGAACCTTATCTGAAACCCACGAGAGGATAGGAACCGACAAAAGTGGCCAAGT GCTAGAATGGAAAGAAGTCATCTATGAAGATGGTGATGAAGACTCAGGAATGGATCCAGAAACATGGGAT GTCTTCCTATCACGGATCAAGGAGGAGAGGGAAGCAGATCCCTCGACATTGAGTGGAATAGCAGGCGTCA AGAAAAGGAAGAGGAAGATGAAAATAGCCATGGCCTTATCTGATCTTGTCATTTATACTAAGGCTGAGAA GTTCCGAAACTTCCAATATTCAAGAGTCTATCAGCAATTTAATGAGACCAATTCGATTGGAGAGTCTCGA GCTCGAAAACTTTCCAAATTGAGAGTCCATGAGTTTATTTTCCACACCGCGGCATTCATCACCAGAGTAT ACCCCAAAATGATGAGAGCAGACTCTTCTAACTTTAACCCTCAAGAGTTTTGGAATGTAGGATGTCAGAT GGTGGCCTTGAACTTTCAAACCCCTGGACTGCCTATGGATTTGCAAAACGGGAAATTTTTGGATAATGGA GGCTCTGGATATATTTTGAAGCCAGACATCCTTAGAGATACAACCCTGGGCTTTAACCCAAATGAACCAG AATATGACGACCATCCAGTTACCCTCACAATCCGAATCATCAGTGGGATCCAGTTGCCTGTTAGCTCATC CTCTAACACGCCTGACATAGTAGTGATCATAGAAGTCTACGGTGTTCCAAACGACCACGTGAAGCAGCAG ACTCGTGTTGTTAAGAATAATGCTTTTAGTCCAAAGTGGAATGAAACATTTACATTTCTTATTCAAGTGC CAGAACTGGCATTGATACGTTTTGTTGTTGAAACTCAACAAGGCTTATTATCAGGAAATGAATTACTCGG GCAGTACACTTTACCAGTTCTTTGCATGAACAAAGGTTATCGTCGTGTTCCTCTGTTTTCCAAATCCGGT GCGAACCTTGAACCTTCCTCACTGTTTATTTATGTTTGGTACTTCAGAGAGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_054066 |
| Insert Size | 1944 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_054066.4, NP_473407.2 |
| RefSeq Size | 2182 bp |
| RefSeq ORF | 1944 bp |
| Locus ID | 114875 |
| UniProt ID | Q8K4D7 |
| Gene Summary | The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. In vitro, hydrolyzes PtdIns(4,5)P2 in a Ca(2+)-dependent manner. Triggers intracellular Ca(2+) oscillations in oocytes solely during M phase and is involved in inducing oocyte activation and initiating embryonic development up to the blastocyst stage. Is therefore a strong candidate for the egg-activating soluble sperm factor that is transferred from the sperm into the egg cytoplasm following gamete membrane fusion. May exert an inhibitory effect on phospholipase-C-coupled processes that depend on calcium ions and protein kinase C, including CFTR trafficking and function.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG223280 | Plcz1 (tGFP-tagged) - Mouse phospholipase C zeta 1 (Plcz1), (10ug) |
CNY 8,824.00 |
|
| MR223280 | Plcz1 (Myc-DDK-tagged) - Mouse phospholipase C, zeta 1 (Plcz1) |
CNY 7,224.00 |
|
| MR223280L3 | Lenti ORF clone of Plcz1 (Myc-DDK-tagged) - Mouse phospholipase C, zeta 1 (Plcz1) |
CNY 6,460.00 |
|
| MR223280L4 | Lenti ORF clone of Plcz1 (mGFP-tagged) - Mouse phospholipase C, zeta 1 (Plcz1) |
CNY 6,460.00 |
