United States
Germany
Japan
United Kingdom
China
Mbd1 (NM_013594) Mouse Untagged Clone
CAT＃: MC219389
Mbd1 (untagged) - Mouse methyl-CpG binding domain protein 1 (Mbd1), (10ug)
CNY 7,510.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | Cxxc3; PCM1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC219389 representing NM_013594
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTGAGTCCTGGCAGGACTGCCCAGCCCTGGGCCCTGGCTGGAAACGCCGAGAGTCCTTTCGAAAGT CAGGGGCCTCGTTTGGACGCTCAGACATCTATTACCAGAGCCCCACAGGAGAAAAGATCCGAAGCAAAGT TGAGCTGACTCGGTACTTGGGCCCTGCATGTGATCTTACCCTGTTTGACTTCAGACAAGGCACCTTATGC CATCCCATTCCCAAGACTCATCCCTTGGCTGTCCCCAGCAAGAAGAAAAAGAAGCCTTCTAAACCAGCCA AGACTAAGAAACAGCAGGTTGGGCTGCAGCGAAGTGAGGTCAGGAGAGAGACTCCACAGGGGGAATATAA GGCCCCTACTGCCACAGCTCTAGCTTCACTATCTGTGTCTGCATCTGCGTCTTCGTCTGCATCTGCATCT GCGTCTTCGTCTGCATCTGCATCTGCATCTGCGTCTTCACATGCACCTGTGTGCTGTGAGAATTGTGGAA TCCACTTTTCATGGGATGGTGTCAAAAGGCAGAGACTTAAGACGTTGTGCAAAGATTGTCGAGCACAGAG AATCGCCTTCAACCGCGAACAGAGAATGTTTAAGCGAGTCGGCTGTGGGGACTGTGCAGCTTGCCTGGTG AAAGAGGACTGTGGGGTGTGCTCCACCTGTCGCCTGCAACTGCCCAGTGATGTGGCCTCAGGGCTTTACT GCAAGTGTGAGCGGAGACGATGCCTGCGGATTATGGAGAAGAGCCGAGGGTGTGGCGTGTGTCGGGGTTG TCAGACCCAAGAGGACTGTGGCCATTGCTGCATCTGTCTTCGCTCTCCCCGCCCTGGTCTCAAGCGCCAG TGGAGGTGTTTGCAACGGCGCTGTTTTTGGGGTAAACGTGATTCTAGTAAGAGAGGCTCGAAGGTAGCGT CTCAGCGTCACTCCCAAGCCCCGCCACTTCCTCCACATCCTGCATCACAGTACACAGAGCCCACAGAGCT GCACATCAGCGACATAGCACCCACATCACCTGCTGAGTTCATCTATTACTGTGTAGACGAGGACGAGGAC GAGCTAAAGCGGCTGCTGCCCAGTGCTGGGTCAGGGTCTGGGGAGGGAGCAGGATTGCGTCCATATCAGA CCCATCAGACCCATCAAAAGAGGCCTGCTTCTGCTCGACAACTCCAACTGAGCTCTCCCTTAAAGGCCCC TTGGGCTGTGGTCACAGCACCACCAGGCCCTGTCCGAGATTCAAGAAAGCAGCAAGCAGGTAGAGGCTCT GTACTGCCCCAGCCTGACACAGACTTTGTGTTTTTACAAGAGGGTACCAGCAGTGCCATGCAGATGCCTG GCACTGCTGCAGCTTGCACAGAAGTCCCAGTACAGGCGGCCCAGTGCTCTGCCCCGAGCTGGGTTGTGGC CTTACCCCAGGTGAAGCAAGAGACGGCAGATGCTCCAGAGGAGTGGACAGCAGTCACGACCTTCCTGACT TCCTCCACGCTGCAGTCTGGCTTCCCTAGCAAGGCTGCAGATCCAGACCTTTCACCTGTGAAACAAGAGC CCCCTGGCCCTGAAGAGGATGGAGAAGAGAAAAAAGATGATGTCTCTGAAACAACCCCAGCAGAGGAGAT AGGAGGGGTTGGCACGCCAGTGATCACGGAGATTTTCAGCCTGGGTGGAACCCGCCTCCGAGATGCAGAA GCCTGGTTGCCAAGGCTACATAAACTGTTAGCAGTAAATGAAAAAGAGTATTTTACTGAGCTGCAGTTGA AAGAAGAAGTTTTGTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_013594 |
| Insert Size | 1767 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_013594.2, NP_038622.2 |
| RefSeq Size | 2672 bp |
| RefSeq ORF | 1767 bp |
| Locus ID | 17190 |
| UniProt ID | Q9Z2E2 |
| Gene Summary | Transcriptional repressor that binds CpG islands in promoters where the DNA is methylated at position 5 of cytosine within CpG dinucleotides. Binding is abolished by the presence of 7-mG that is produced by DNA damage by methylmethanesulfonate (MMS). Acts as transcriptional repressor and plays a role in gene silencing by recruiting AFT7IP, which in turn recruits factors such as the histone methyltransferase SETDB1. Probably forms a complex with SETDB1 and ATF7IP that represses transcription and couples DNA methylation and histone 'Lys-9' trimethylation. Isoform 1 can also repress transcription from unmethylated promoters.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MR209180 | Mbd1 (Myc-DDK-tagged) - Mouse methyl-CpG binding domain protein 1 (Mbd1) |
CNY 4,376.00 |
|
| MR209180L3 | Lenti ORF clone of Mbd1 (Myc-DDK-tagged) - Mouse methyl-CpG binding domain protein 1 (Mbd1) |
CNY 7,130.00 |
|
| MR209180L4 | Lenti ORF clone of Mbd1 (mGFP-tagged) - Mouse methyl-CpG binding domain protein 1 (Mbd1) |
CNY 7,130.00 |
