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Smad4 (NM_008540) Mouse Untagged Clone
CAT＃: MC218774
Smad4 (untagged) - Mouse MAD homolog 4 (Drosophila) (Smad4), (10ug)
CNY 4,112.00
CNY 7,030.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | AW743858; D18Wsu70e; DPC4; Madh4 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>NCBI ORF sequence for NM_008540, the custom clone sequence may differ by one or more nucleotides
ATGGACAATATGTCTATAACAAATACACCAACAAGTAACGATGCCTGTCTGAGCATTGTACATAGTTTGA TGTGTCATAGACAAGGTGGGGAAAGTGAAACCTTTGCAAAAAGAGCAATTGAGAGTTTGGTAAAGAAGCT GAAAGAGAAAAAAGATGAATTGGATTCTTTAATAACAGCTATAACTACAAATGGAGCTCATCCTAGCAAG TGTGTCACCATACAGAGAACATTGGATGGACGACTTCAGGTGGCTGGTCGGAAAGGATTTCCTCATGTGA TCTATGCCCGTCTGTGGAGGTGGCCTGATCTACACAAGAATGAACTAAAGCATGTTAAATATTGTCAGTA TGCGTTTGACTTAAAATGTGACAGTGTCTGTGTGAATCCATATCACTATGAGCGGGTTGTCTCACCTGGA ATTGATCTCTCAGGATTAACACTGCAGAGTAATGCTCCAAGTATGTTAGTGAAGGATGAGTACGTTCACG ACTTTGAAGGACAGCCGTCCTTACCCACTGAAGGACATTCGATTCAAACCATCCAACACCCGCCAAGTAA TCGCGCATCAACGGAGACGTACAGCGCCCCGGCTCTGTTAGCCCCGGCAGAGTCTAACGCCACCAGCACC ACCAACTTCCCCAACATTCCTGTGGCTTCCACAAGTCAGCCGGCCAGTATTCTGGCGGGCAGCCATAGTG AAGGACTGTTGCAGATAGCTTCAGGGCCTCAGCCAGGACAGCAGCAGAATGGATTTACTGCTCAGCCAGC TACTTACCATCATAACAGCACTACCACCTGGACTGGAAGTAGGACTGCACCATACACACCTAATTTGCCT CACCACCAAAACGGCCATCTTCAGCACCACCCGCCTATGCCGCCCCATCCTGGACATTACTGGCCAGTTC ACAATGAGCTTGCATTCCAGCCTCCCATTTCCAATCATCCTGCTCCTGAGTACTGGTGCTCCATTGCTTA CTTTGAAATGGACGTTCAGGTAGGAGAGACGTTTAAGGTCCCTTCAAGCTGCCCTGTTGTGACTGTGGAT GGCTATGTGGATCCTTCGGGAGGAGATCGCTTTTGCTTGGGTCAACTCTCCAATGTCCACAGGACAGAAG CGATTGAGAGAGCGAGGTTGCACATAGGCAAAGGAGTGCAGTTGGAATGTAAAGGTGAAGGTGACGTTTG GGTCAGGTGCCTTAGTGACCACGCGGTCTTTGTACAGAGTTACTACCTGGACAGAGAAGCTGGCCGAGCA CCTGGCGACGCTGTTCATAAGATCTACCCAAGCGCGTATATAAAGGTCTTTGATCTGCGGCAGTGTCACC GGCAGATGCAGCAACAGGCGGCCACTGCGCAAGCTGCAGCTGCTGCTCAGGCGGCGGCCGTGGCAGGGAA CATCCCTGGCCCTGGGTCCGTGGGTGGAATAGCTCCAGCCATCAGTCTGTCTGCTGCTGCTGGCATCGGT GTGGATGACCTCCGGCGATTGTGCATTCTCAGGATGAGCTTTGTGAAGGGCTGGGGCCCAGACTACCCCA GGCAGAGCATCAAGGAAACCCCGTGCTGGATTGAGATTCACCTTCACCGAGCTCTGCAGCTCTTGGATGA AGTCCTGCACACCATGCCCATTGCGGACCCACAGCCTTTAGACTGA |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_008540 |
| Insert Size | 1656 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_008540.2, NP_032566.2 |
| RefSeq Size | 3361 bp |
| RefSeq ORF | 1656 bp |
| Locus ID | 17128 |
| UniProt ID | P97471 |
| Gene Summary | Common SMAD (co-SMAD) is the coactivator and mediator of signal transduction by TGF-beta (transforming growth factor). Component of the heterotrimeric SMAD2/SMAD3-SMAD4 complex that forms in the nucleus and is required for the TGF-mediated signaling. Promotes binding of the SMAD2/SMAD4/FAST-1 complex to DNA and provides an activation function required for SMAD1 or SMAD2 to stimulate transcription. Component of the multimeric SMAD3/SMAD4/JUN/FOS complex which forms at the AP1 promoter site; required for synergistic transcriptional activity in response to TGF-beta. May act as a tumor suppressor. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator (By similarity). Acts synergistically with SMAD1 and YY1 in bone morphogenetic protein (BMP)-mediated cardiac-specific gene expression (PubMed:15329343). Binds to SMAD binding elements (SBEs) (5'-GTCT/AGAC-3') within BMP response element (BMPRE) of cardiac activating regions (PubMed:15329343). In muscle physiology, plays a central role in the balance between atrophy and hypertrophy. When recruited by MSTN, promotes atrophy response via phosphorylated SMAD2/4. MSTN decrease causes SMAD4 release and subsequent recruitment by the BMP pathway to promote hypertrophy via phosphorylated SMAD1/5/8.[UniProtKB/Swiss-Prot Function] |
Documents
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| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MR208755 | Smad4 (Myc-DDK-tagged) - Mouse MAD homolog 4 (Drosophila) (Smad4) |
CNY 6,168.00 |
|
| MR208755L3 | Lenti ORF clone of Smad4 (Myc-DDK-tagged) - Mouse MAD homolog 4 (Drosophila) (Smad4) |
CNY 8,568.00 |
|
| MR208755L4 | Lenti ORF clone of Smad4 (mGFP-tagged) - Mouse MAD homolog 4 (Drosophila) (Smad4) |
CNY 8,568.00 |
