Rad18 (BC011120) Mouse Untagged Clone
CAT#: MC218342
Rad18 (untagged) - Mouse RAD18 homolog (S. cerevisiae) (cDNA clone MGC:19000 IMAGE:4014013), (10ug)
CNY 6,940.00
Product images
Specifications
Product Data | |
Type | Mouse Untagged Clone |
Tag | Tag Free |
Synonyms | 2810024C04Rik; Rad18sc |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>BC011120
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGGTCCTGGCCGAGCCGCGATGGCCTCCGGGGTTGGCGGTAATGAAGACAATAGATGACTTGCTGC GCTGTGGGATTTGTTTTGAGTATTTCAACATTGCAGTGATAATCCCCCAGTGCTCTCACAACTATTGTTC ACTCTGTATAAGAAAGTTTTTATCCTATAAAACTCAGTGCCCAACTTGTTGCGTGGCAGTAACGGAGCCA GACCTGAGAAATAATCGCCTCTTAGATGAACTGGTAAAAAGCATGAATTTTGCACGGACTCACCTGTTGC AGTTTGCTTTAGAGTCACCACCCATATCTCCTGTGTCTTCCACCTCAAAGAAGGTTGTTGTTAAAGTGCA TAATGCTGACGCCGCCCAACACCCTGTCAAACAGGGGAACAGGTTAATGGATAAGTTCCTGATTAGAGAA ACTGGTGACTGTGTATTTGAGTTGTTGGGAAAAGAAAATGAGAGGAAATTCAGCCCTCAAAAAGAGCTAA GCACCTCTGCTGAGATTAAAGAGACAAGTCTCCTAGGAAAGCCGGTACTGGGTCTCTCAGATGCTAATGG TCCTGTGACTCCCTCTACATCCACTATGAAACTGGATACTAAAGTGTCTTGTCCTGTTTGTGGGGTCAGC ATTCCAGAAAATCATATCAATAAGCATTTAGACAGTTGTCTATCACGTGAAGAGAAGAAGGAGAGCCTGC GAAGTTCTGCTCACAAAAGGAAGCCGTTGCCCAAAACTGTATATAACTTGCTCTCTGATCGTGATTTAAA GAAAAAGCTGAAACAGTATGGCTTATCTGTTCAAGGAAACAAACAGCAGCTTATTAAAAGGCATCAGGAA TTTGTGCACATGTATAATGCCCAGTGCGATGCTTTGCATCCTAAATCAGGTCATGGTTTTTACAAAGAAC CAAACAGAGAAGGAAATTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCTGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
Restriction Sites | SgfI-MluI |
ACCN | BC011120 |
Insert Size | 930 bp |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | BC011120, AAH11120 |
RefSeq Size | 1482 bp |
RefSeq ORF | 929 bp |
Locus ID | 58186 |
Gene Summary | E3 ubiquitin-protein ligase involved in postreplication repair of UV-damaged DNA. Postreplication repair functions in gap-filling of a daughter strand on replication of damaged DNA. Associates to the E2 ubiquitin conjugating enzyme UBE2B to form the UBE2B-RAD18 ubiquitin ligase complex involved in mono-ubiquitination of DNA-associated PCNA on 'Lys-164'. Has ssDNA binding activity.[UniProtKB/Swiss-Prot Function] |
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Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MG204378 | Rad18 (tGFP-tagged) - Mouse RAD18 homolog (S. cerevisiae) (cDNA clone MGC:19000 IMAGE:4014013) |
CNY 4,000.00 |
|
MR204378 | Rad18 (Myc-DDK-tagged) - Mouse RAD18 homolog (S. cerevisiae) (cDNA clone MGC:19000 IMAGE:4014013) |
CNY 2,400.00 |
|
MR204378L3 | Lenti ORF clone of Rad18 (Myc-DDK-tagged) - Mouse RAD18 homolog (S. cerevisiae) (cDNA clone MGC:19000 IMAGE:4014013) |
CNY 4,750.00 |
|
MR204378L4 | Lenti ORF clone of Rad18 (mGFP-tagged) - Mouse RAD18 homolog (S. cerevisiae) (cDNA clone MGC:19000 IMAGE:4014013) |
CNY 4,750.00 |