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Rad18 (BC011120) Mouse Tagged ORF Clone
CAT＃: MG204378
- TrueORF®
Rad18 (tGFP-tagged) - Mouse RAD18 homolog (S. cerevisiae) (cDNA clone MGC:19000 IMAGE:4014013)
ORF Plasmid: DDK
"BC011120" in other vectors (4)
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CNY 4,000.00
Product images
CNY 1,000.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 2810024C04Rik; Rad18sc |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG204378 representing BC011120
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGGTCCTGGCCGAGCCGCGATGGCCTCCGGGGTTGGCGGTAATGAAGACAATAGATGACTTGCTGC GCTGTGGGATTTGTTTTGAGTATTTCAACATTGCAGTGATAATCCCCCAGTGCTCTCACAACTATTGTTC ACTCTGTATAAGAAAGTTTTTATCCTATAAAACTCAGTGCCCAACTTGTTGCGTGGCAGTAACGGAGCCA GACCTGAGAAATAATCGCCTCTTAGATGAACTGGTAAAAAGCATGAATTTTGCACGGACTCACCTGTTGC AGTTTGCTTTAGAGTCACCACCCATATCTCCTGTGTCTTCCACCTCAAAGAAGGTTGTTGTTAAAGTGCA TAATGCTGACGCCGCCCAACACCCTGTCAAACAGGGGAACAGGTTAATGGATAAGTTCCTGATTAGAGAA ACTGGTGACTGTGTATTTGAGTTGTTGGGAAAAGAAAATGAGAGGAAATTCAGCCCTCAAAAAGAGCTAA GCACCTCTGCTGAGATTAAAGAGACAAGTCTCCTAGGAAAGCCGGTACTGGGTCTCTCAGATGCTAATGG TCCTGTGACTCCCTCTACATCCACTATGAAACTGGATACTAAAGTGTCTTGTCCTGTTTGTGGGGTCAGC ATTCCAGAAAATCATATCAATAAGCATTTAGACAGTTGTCTATCACGTGAAGAGAAGAAGGAGAGCCTGC GAAGTTCTGCTCACAAAAGGAAGCCGTTGCCCAAAACTGTATATAACTTGCTCTCTGATCGTGATTTAAA GAAAAAGCTGAAACAGTATGGCTTATCTGTTCAAGGAAACAAACAGCAGCTTATTAAAAGGCATCAGGAA TTTGTGCACATGTATAATGCCCAGTGCGATGCTTTGCATCCTAAATCAGGTCATGGTTTTTACAAAGAAC CAAACAGAGAAGGAAAT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG204378 representing BC011120
Red=Cloning site Green=Tags(s) MEVLAEPRWPPGLAVMKTIDDLLRCGICFEYFNIAVIIPQCSHNYCSLCIRKFLSYKTQCPTCCVAVTEP DLRNNRLLDELVKSMNFARTHLLQFALESPPISPVSSTSKKVVVKVHNADAAQHPVKQGNRLMDKFLIRE TGDCVFELLGKENERKFSPQKELSTSAEIKETSLLGKPVLGLSDANGPVTPSTSTMKLDTKVSCPVCGVS IPENHINKHLDSCLSREEKKESLRSSAHKRKPLPKTVYNLLSDRDLKKKLKQYGLSVQGNKQQLIKRHQE FVHMYNAQCDALHPKSGHGFYKEPNREGN TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC011120 |
| ORF Size | 927 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC011120.1 |
| RefSeq Size | 1482 bp |
| RefSeq ORF | 929 bp |
| Locus ID | 58186 |
| Gene Summary | E3 ubiquitin-protein ligase involved in postreplication repair of UV-damaged DNA. Postreplication repair functions in gap-filling of a daughter strand on replication of damaged DNA. Associates to the E2 ubiquitin conjugating enzyme UBE2B to form the UBE2B-RAD18 ubiquitin ligase complex involved in mono-ubiquitination of DNA-associated PCNA on 'Lys-164'. Has ssDNA binding activity.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC218342 | Rad18 (untagged) - Mouse RAD18 homolog (S. cerevisiae) (cDNA clone MGC:19000 IMAGE:4014013), (10ug) |
CNY 6,940.00 |
|
| MR204378 | Rad18 (Myc-DDK-tagged) - Mouse RAD18 homolog (S. cerevisiae) (cDNA clone MGC:19000 IMAGE:4014013) |
CNY 2,400.00 |
|
| MR204378L3 | Lenti ORF clone of Rad18 (Myc-DDK-tagged) - Mouse RAD18 homolog (S. cerevisiae) (cDNA clone MGC:19000 IMAGE:4014013) |
CNY 4,750.00 |
|
| MR204378L4 | Lenti ORF clone of Rad18 (mGFP-tagged) - Mouse RAD18 homolog (S. cerevisiae) (cDNA clone MGC:19000 IMAGE:4014013) |
CNY 4,750.00 |
