Atat1 (NM_028476) Mouse Untagged Clone
CAT#: MC211392
Atat1 (untagged) - Mouse alpha tubulin acetyltransferase 1 (Atat1), transcript variant 3, (10ug)
CNY 3,990.00
Product images
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Specifications
Product Data | |
Type | Mouse Untagged Clone |
Tag | Tag Free |
Synonyms | 0610011P08Rik; 2610008K08Rik; 2610110G12Rik; 3110080J08Rik; Mec17; TAT |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MC211392 representing NM_028476
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGTTCCCGTTCGATGTGGATGCGCTGTTCCCGGAGCGGATCACCGTGCTGGACCAGCACCTGCGGC CTCCGGCCCGCCGACCCGGAACCACAACGCCGGCCCGTGTGGATCTGCAGCAGCAAATCATGACTATTGT AGATGAGCTGGGCAAGGCTTCTGCCAAGGCGCAGCACCTCCCTGCACCCATCACCAGCGCTTTGAGGATG CAAAGCAACCGGCACGTTATTTACATACTGAAGGACACCTCAGCCCGACCGGCAGGGAAAGGAGCCATTA TTGGTTTCCTCAAAGTTGGATACAAGAAGCTCTTTGTACTGGATGACCGGGAGGCTCACAATGAGGTAGA ACCCCTTTGCATTCTGGACTTTTACATCCACGAGTCGGTGCAACGGCATGGCCACGGGCGAGAACTTTTT CAGCATATGTTACAGAAAGAGCGAGTGGAGCCCCACCAACTGGCCATTGACCGACCATCGCCGAAGCTGC TCAAGTTCCTGAATAAGCACTACAACCTGGAGACCACAGTCCCACAGGTGAACAACTTTGTCATCTTTGA AGGCTTCTTTGCCCATCAGCACCCTCCAGCACGGAAGCTGCCACCAAAAAGAGCAGAGGGAGACATTAAG CCATACTCTTCCAGTGACAGAGAATTCCTGAAGGTAGCTGTGGAGCCTCCTTGGCCCCTGAACAGGGCCC CTCGGCGTGCCACACCTCCAGCCCACCCACCTCCACGTTCTAGCAGCCTGGGCAACTCACCGGATCGGGG TCCCCTTCGGCCCTTTGTTCCAGAGCAGGAGCTGCTTCGCTCCCTGCGTCTCTGTCCCCCACACCCTACT GCACGCCTTCTGCTGGCCACTGACCCTGGAGGCAGCCCAGCCCAGCGCAGACGCACCAGCTCCCTTCCCC GATCTGATGAGAGTCGATACTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
Restriction Sites | SgfI-MluI |
ACCN | NM_028476 |
Insert Size | 933 bp |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_028476.4, NP_082752.3 |
RefSeq Size | 1999 bp |
RefSeq ORF | 933 bp |
Locus ID | 73242 |
UniProt ID | Q8K341 |
Gene Summary | Specifically acetylates 'Lys-40' in alpha-tubulin on the lumenal side of microtubules. Promotes microtubule destabilization and accelerates microtubule dynamics; this activity may be independent of acetylation activity. Acetylates alpha-tubulin with a slow enzymatic rate, due to a catalytic site that is not optimized for acetyl transfer. Enters the microtubule through each end and diffuses quickly throughout the lumen of microtubules. Acetylates only long/old microtubules because of its slow acetylation rate since it does not have time to act on dynamically unstable microtubules before the enzyme is released. Required for normal sperm flagellar function. Promotes directional cell locomotion and chemotaxis, through AP2A2-dependent acetylation of alpha-tubulin at clathrin-coated pits that are concentrated at the leading edge of migrating cells. May facilitate primary cilium assembly.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (3) lacks an internal in-frame coding exon and a few of 3' exons but has an alternate 3' exon, as compared to variant 1. The resulting isoform (3) lacks an internal segment and has a shorter and different C-terminus, as compared to isoform 1. It should be noted that this variant contains an overlapping upstream ORF that may interfere with the translation of the annotated CDS. |
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Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MG222770 | Atat1 (tGFP-tagged) - Mouse RIKEN cDNA 2610110G12 gene (2610110G12Rik) transcript variant 3, (10ug) |
CNY 3,420.00 |
|
MR222770 | Atat1 (Myc-DDK-tagged) - Mouse alpha tubulin acetyltransferase 1 (Atat1), transcript variant 3 |
CNY 3,140.00 |
|
MR222770L3 | Lenti ORF clone of Atat1 (Myc-DDK-tagged) - Mouse alpha tubulin acetyltransferase 1 (Atat1), transcript variant 3 |
CNY 5,040.00 |
|
MR222770L4 | Lenti ORF clone of Atat1 (mGFP-tagged) - Mouse alpha tubulin acetyltransferase 1 (Atat1), transcript variant 3 |
CNY 5,040.00 |