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Atat1 (NM_028476) Mouse Tagged ORF Clone
CAT＃: MG222770
- TrueORF®
Atat1 (tGFP-tagged) - Mouse RIKEN cDNA 2610110G12 gene (2610110G12Rik) transcript variant 3, (10ug)
ORF Plasmid: DDK
"NM_028476" in other vectors (4)
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CNY 3,420.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 0610011P08Rik; 2610008K08Rik; 2610110G12Rik; 3110080J08Rik; Mec17; TAT |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG222770 representing NM_028476
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGTTCCCGTTCGATGTGGATGCGCTGTTCCCGGAGCGGATCACCGTGCTGGACCAGCACCTGCGGC CTCCGGCCCGCCGACCCGGAACCACAACGCCGGCCCGTGTGGATCTGCAGCAGCAAATCATGACTATTGT AGATGAGCTGGGCAAGGCTTCTGCCAAGGCGCAGCACCTCCCTGCACCCATCACCAGCGCTTTGAGGATG CAAAGCAACCGGCACGTTATTTACATACTGAAGGACACCTCAGCCCGACCGGCAGGGAAAGGAGCCATTA TTGGTTTCCTCAAAGTTGGATACAAGAAGCTCTTTGTACTGGATGACCGGGAGGCTCACAATGAGGTAGA ACCCCTTTGCATTCTGGACTTTTACATCCACGAGTCGGTGCAACGGCATGGCCACGGGCGAGAACTTTTT CAGCATATGTTACAGAAAGAGCGAGTGGAGCCCCACCAACTGGCCATTGACCGACCATCGCCGAAGCTGC TCAAGTTCCTGAATAAGCACTACAACCTGGAGACCACAGTCCCACAGGTGAACAACTTTGTCATCTTTGA AGGCTTCTTTGCCCATCAGCACCCTCCAGCACGGAAGCTGCCACCAAAAAGAGCAGAGGGAGACATTAAG CCATACTCTTCCAGTGACAGAGAATTCCTGAAGGTAGCTGTGGAGCCTCCTTGGCCCCTGAACAGGGCCC CTCGGCGTGCCACACCTCCAGCCCACCCACCTCCACGTTCTAGCAGCCTGGGCAACTCACCGGATCGGGG TCCCCTTCGGCCCTTTGTTCCAGAGCAGGAGCTGCTTCGCTCCCTGCGTCTCTGTCCCCCACACCCTACT GCACGCCTTCTGCTGGCCACTGACCCTGGAGGCAGCCCAGCCCAGCGCAGACGCACCAGCTCCCTTCCCC GATCTGATGAGAGTCGATAC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG222770 representing NM_028476
Red=Cloning site Green=Tags(s) MEFPFDVDALFPERITVLDQHLRPPARRPGTTTPARVDLQQQIMTIVDELGKASAKAQHLPAPITSALRM QSNRHVIYILKDTSARPAGKGAIIGFLKVGYKKLFVLDDREAHNEVEPLCILDFYIHESVQRHGHGRELF QHMLQKERVEPHQLAIDRPSPKLLKFLNKHYNLETTVPQVNNFVIFEGFFAHQHPPARKLPPKRAEGDIK PYSSSDREFLKVAVEPPWPLNRAPRRATPPAHPPPRSSSLGNSPDRGPLRPFVPEQELLRSLRLCPPHPT ARLLLATDPGGSPAQRRRTSSLPRSDESRY TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_028476 |
| ORF Size | 930 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_028476.4, NP_082752.3 |
| RefSeq Size | 1999 bp |
| RefSeq ORF | 933 bp |
| Locus ID | 73242 |
| UniProt ID | Q8K341 |
| Gene Summary | Specifically acetylates 'Lys-40' in alpha-tubulin on the lumenal side of microtubules. Promotes microtubule destabilization and accelerates microtubule dynamics; this activity may be independent of acetylation activity. Acetylates alpha-tubulin with a slow enzymatic rate, due to a catalytic site that is not optimized for acetyl transfer. Enters the microtubule through each end and diffuses quickly throughout the lumen of microtubules. Acetylates only long/old microtubules because of its slow acetylation rate since it does not have time to act on dynamically unstable microtubules before the enzyme is released. Required for normal sperm flagellar function. Promotes directional cell locomotion and chemotaxis, through AP2A2-dependent acetylation of alpha-tubulin at clathrin-coated pits that are concentrated at the leading edge of migrating cells. May facilitate primary cilium assembly.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC211392 | Atat1 (untagged) - Mouse alpha tubulin acetyltransferase 1 (Atat1), transcript variant 3, (10ug) |
CNY 3,990.00 |
|
| MR222770 | Atat1 (Myc-DDK-tagged) - Mouse alpha tubulin acetyltransferase 1 (Atat1), transcript variant 3 |
CNY 3,140.00 |
|
| MR222770L3 | Lenti ORF clone of Atat1 (Myc-DDK-tagged) - Mouse alpha tubulin acetyltransferase 1 (Atat1), transcript variant 3 |
CNY 5,040.00 |
|
| MR222770L4 | Lenti ORF clone of Atat1 (mGFP-tagged) - Mouse alpha tubulin acetyltransferase 1 (Atat1), transcript variant 3 |
CNY 5,040.00 |
