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Skp2 (NM_145468) Mouse Untagged Clone
CAT＃: MC209794
Skp2 (untagged) - Mouse S-phase kinase-associated protein 2 (p45) (Skp2), transcript variant 2, (10ug)
CNY 3,990.00
| Cited in 5 publications. |
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | FBXL1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC209794 representing NM_145468
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCATAGGAAGCACCTTCAGGAGATTCCGGACCAGAGTGGCAACGTCACCACCAGCTTCACGTGGGGAT GGGATTCCAGCAAGACTTCTGAACTGCTATCAGGCATGGGTGTCTCGGCCTTGGAGAAGGAGGAGGTGGA CAGTGAGAACATCCCACATGGACTGCTCTCAAACCTCGGCCACCCCCAGAGCCCTCCAAGGAAACGAGTC AAGGGCAAAGGGAGTGACAAAGACTTTGTGATCATCCGTCGGCCGAAGCTTAGTCGGGAGAACTTTCCAG GTGTCTCCTGGGACTCCCTTCCAGATGAGCTGCTCCTTGGGATCTTTTCCTGTCTGTGCCTCCCTGAGCT GCTGAGAGTCTCGGGCGTTTGCAAGAGGTGGTACCGCCTCTCGCTCGATGAGTCTCTCTGGCAGTCCCTC GACCTCGCGGGTAAAAATCTGCACCCAGACGTGACTGTGCGCTTGCTCTCCCGCGGGGTGGTCGCCTTCC GCTGCCCTCGGTCCTTTATGGAGCAGCCGCTGGGTGAAAGCTTCAGCTCTTTCCGGGTACAGCACATGGA CCTGTCGAACTCAGTGATAAATGTGTCGAACCTCCATAAGATTCTGTCCGAGTGCTCCAAGCTGCAGAAT CTAAGCCTGGAAGGCCTGCAGCTCTCAGACCCCATTGTCAAGACTCTTGCACAGAATGAAAACTTGGTGC GACTAAACCTTTGTGGGTGCTCTGGGTTTTCTGAATCTGCCGTGGCGACTCTGCTAAGCAGCTGCTCCAG ACTGGATGAGCTAAATCTCTCCTGGTGCTTTGACTTCACTGAAAAGCACGTGCAAGCGGCTGTGGCACAT TTACCAAACACCATCACCCAGCTGAACCTCAGCGGCTACCGAAAGAACCTCCAGAAAACAGATCTTTGTA CCATAATTAAACGATGCCCCAACCTCATCCGCCTCGACTTAAGTGACAGTATCATGCTAAAGAATGACTG CTTTCCAGAATTTTTTCAACTCAACTACCTCCAACACCTCTCGCTCAGCCGGTGCTATGATATAATACCT GATACTCTACTGTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_145468 |
| Insert Size | 1065 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_145468.1, NP_663443.1 |
| RefSeq Size | 6196 bp |
| RefSeq ORF | 1065 bp |
| Locus ID | 27401 |
| Gene Summary | Substrate recognition component of the SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins involved in cell cycle progression, signal transduction and transcription. The SCF complex provides substrate specificity and interacts with both, the E2 ubiquitin-conjugating enzyme and the substrate. Specifically recognizes phosphorylated CDKN1B/p27kip and is involved in regulation of G1/S transition. Degradation of CDKN1B/p27kip also requires CKS1. Promotes ubiquitination and destruction of CDH1 in a CK1-Dependent Manner, thereby regulating cell migration (By similarity).[UniProtKB/Swiss-Prot Function] |
Citations (5)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Tanshinone IIA regulates colorectal cancer apoptosis via attenuation of Parkin‑mediated mitophagy by suppressing AMPK/Skp2 pathways.
,null,
Molecular medicine reports
,PubMed ID 29845197
[Skp2]
|
|
NKX6.3 Is a Transcription Factor for Wnt/β-catenin and Rho-GTPase Signaling-Related Genes to Suppress Gastric Cancer Progression
,null,
EBioMedicine
,PubMed ID 27333045
[Skp2]
|
|
Mechanisms in cardiac fibroblast growth: an obligate role for Skp2 and FOXO3a in ERK1/2 MAPK-dependent regulation of p27kip1.
,null,
American journal of physiology. Heart and circulatory physiology
,PubMed ID 24441545
[Skp2]
|
|
Deacetylation of FOXO3 by SIRT1 or SIRT2 leads to Skp2-mediated FOXO3 ubiquitination and degradation.
,null,
Oncogene
,PubMed ID 21841822
[Skp2]
|
|
SKP2 and CKS1 promote degradation of cell cycle regulators and are associated with hepatocellular carcinoma prognosis.
,null,
Gastroenterology
,PubMed ID 19686743
[Skp2]
|
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG225316 | Skp2 (tGFP-tagged) - Mouse S-phase kinase-associated protein 2 (p45) (Skp2) transcript variant 2, (10ug) |
CNY 3,900.00 |
|
| MR225316 | Skp2 (Myc-DDK-tagged) - Mouse S-phase kinase-associated protein 2 (p45) (Skp2), transcript variant 2 |
CNY 3,520.00 |
|
| MR225316L3 | Lenti ORF clone of Skp2 (Myc-DDK-tagged) - Mouse S-phase kinase-associated protein 2 (p45) (Skp2), transcript variant 2 |
CNY 5,420.00 |
|
| MR225316L4 | Lenti ORF clone of Skp2 (mGFP-tagged) - Mouse S-phase kinase-associated protein 2 (p45) (Skp2), transcript variant 2 |
CNY 5,420.00 |
