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Skp2 (NM_145468) Mouse Tagged ORF Clone
CAT＃: MR225316
- TrueORF®
Skp2 (Myc-DDK-tagged) - Mouse S-phase kinase-associated protein 2 (p45) (Skp2), transcript variant 2
ORF Plasmid: tGFP
"NM_145468" in other vectors (4)
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CNY 3,520.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | FBXL1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR225316 representing NM_145468
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCATAGGAAGCACCTTCAGGAGATTCCGGACCAGAGTGGCAACGTCACCACCAGCTTCACGTGGGGAT GGGATTCCAGCAAGACTTCTGAACTGCTATCAGGCATGGGTGTCTCGGCCTTGGAGAAGGAGGAGGTGGA CAGTGAGAACATCCCACATGGACTGCTCTCAAACCTCGGCCACCCCCAGAGCCCTCCAAGGAAACGAGTC AAGGGCAAAGGGAGTGACAAAGACTTTGTGATCATCCGTCGGCCGAAGCTTAGTCGGGAGAACTTTCCAG GTGTCTCCTGGGACTCCCTTCCAGATGAGCTGCTCCTTGGGATCTTTTCCTGTCTGTGCCTCCCTGAGCT GCTGAGAGTCTCGGGCGTTTGCAAGAGGTGGTACCGCCTCTCGCTCGATGAGTCTCTCTGGCAGTCCCTC GACCTCGCGGGTAAAAATCTGCACCCAGACGTGACTGTGCGCTTGCTCTCCCGCGGGGTGGTCGCCTTCC GCTGCCCTCGGTCCTTTATGGAGCAGCCGCTGGGTGAAAGCTTCAGCTCTTTCCGGGTACAGCACATGGA CCTGTCGAACTCAGTGATAAATGTGTCGAACCTCCATAAGATTCTGTCCGAGTGCTCCAAGCTGCAGAAT CTAAGCCTGGAAGGCCTGCAGCTCTCAGACCCCATTGTCAAGACTCTTGCACAGAATGAAAACTTGGTGC GACTAAACCTTTGTGGGTGCTCTGGGTTTTCTGAATCTGCCGTGGCGACTCTGCTAAGCAGCTGCTCCAG ACTGGATGAGCTAAATCTCTCCTGGTGCTTTGACTTCACTGAAAAGCACGTGCAAGCGGCTGTGGCACAT TTACCAAACACCATCACCCAGCTGAACCTCAGCGGCTACCGAAAGAACCTCCAGAAAACAGATCTTTGTA CCATAATTAAACGATGCCCCAACCTCATCCGCCTCGACTTAAGTGACAGTATCATGCTAAAGAATGACTG CTTTCCAGAATTTTTTCAACTCAACTACCTCCAACACCTCTCGCTCAGCCGGTGCTATGATATAATACCT GATACTCTACTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR225316 representing NM_145468
Red=Cloning site Green=Tags(s) MHRKHLQEIPDQSGNVTTSFTWGWDSSKTSELLSGMGVSALEKEEVDSENIPHGLLSNLGHPQSPPRKRV KGKGSDKDFVIIRRPKLSRENFPGVSWDSLPDELLLGIFSCLCLPELLRVSGVCKRWYRLSLDESLWQSL DLAGKNLHPDVTVRLLSRGVVAFRCPRSFMEQPLGESFSSFRVQHMDLSNSVINVSNLHKILSECSKLQN LSLEGLQLSDPIVKTLAQNENLVRLNLCGCSGFSESAVATLLSSCSRLDELNLSWCFDFTEKHVQAAVAH LPNTITQLNLSGYRKNLQKTDLCTIIKRCPNLIRLDLSDSIMLKNDCFPEFFQLNYLQHLSLSRCYDIIP DTLL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_145468 |
| ORF Size | 1062 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_145468.1, NP_663443.1 |
| RefSeq Size | 6196 bp |
| RefSeq ORF | 1065 bp |
| Locus ID | 27401 |
| MW | 227.2 kDa |
| Gene Summary | Substrate recognition component of the SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins involved in cell cycle progression, signal transduction and transcription. The SCF complex provides substrate specificity and interacts with both, the E2 ubiquitin-conjugating enzyme and the substrate. Specifically recognizes phosphorylated CDKN1B/p27kip and is involved in regulation of G1/S transition. Degradation of CDKN1B/p27kip also requires CKS1. Promotes ubiquitination and destruction of CDH1 in a CK1-Dependent Manner, thereby regulating cell migration (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC209794 | Skp2 (untagged) - Mouse S-phase kinase-associated protein 2 (p45) (Skp2), transcript variant 2, (10ug) |
CNY 3,990.00 |
|
| MG225316 | Skp2 (tGFP-tagged) - Mouse S-phase kinase-associated protein 2 (p45) (Skp2) transcript variant 2, (10ug) |
CNY 3,900.00 |
|
| MR225316L3 | Lenti ORF clone of Skp2 (Myc-DDK-tagged) - Mouse S-phase kinase-associated protein 2 (p45) (Skp2), transcript variant 2 |
CNY 5,420.00 |
|
| MR225316L4 | Lenti ORF clone of Skp2 (mGFP-tagged) - Mouse S-phase kinase-associated protein 2 (p45) (Skp2), transcript variant 2 |
CNY 5,420.00 |
