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Chst4 (NM_011998) Mouse Untagged Clone
CAT＃: MC207463
Chst4 (untagged) - Mouse carbohydrate (chondroitin 6/keratan) sulfotransferase 4 (Chst4), (10ug)
CNY 3,990.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | Gn6st-2; GST-3; HEC-GlcNAc6ST |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC207463 representing NM_011998
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATGCTGTTGAAGAAAGGGAGGCTGCTGATGTTCCTGGGTTCCCAGGTCATCGTTGTAGCTCTCTTCA TCCATATGTCCGTCCACAGACACCTTTCCCAGAGGGAGGAGTCCAGGAGGCCCGTGCATGTGCTGGTGCT GTCTTCCTGGCGGTCAGGATCCTCTTTTGTGGGACAGCTTTTCGGGCAGCACCCGGATGTGTTCTACCTG ATGGAGCCTGCCTGGCATGTGTGGATGACTTTCACCAGCAGCACAGCCTGGAAGCTGCACATGGCTGTGC GGGATCTTCTGCGTTCCGTCTTCCTGTGTGACATGAGCGTCTTTGATGCCTACATGAACCCAGGCCCCCG GAAACAGTCCAGCCTCTTCCAGTGGGAGCAAAGCCGGGCCCTGTGCTCAGCGCCTGTGTGTGACTTCTTC CCTGCCCACGAGATCAGCTCACCCAAGCACTGCAAGCTGCTCTGCGGTCAGCAGCCCTTTGATATGGTGG AGAAGGCCTGCCGCTCTCACGGCTTCGTGGTACTCAAGGAGGTGCGTTTTCTCAGCCTGCAGGCCCTCTA TCCACTACTCACGGACCCTTCCCTCAACCTGCACGTCGTGCACCTGGTCCGAGACCCCCGGGCCGTGTTC CGATCCCGGGAGCACACCACCATAGAACTCATGGTTGACAGTCATATTGTGCTAGGGCAGCATTTGGAAA CGATCAAGGAGGAAGACCAGCCCTATTATGCCATGAAGATCATCTGCAAAAGCCAGGTGGACATAGTCAA GGCCATCCAAACCCTCCCTGAAGCTCTGCAGCAGCGCTACCTGTTCCTGAGGTATGAGGACCTGGTTCGG GCACCCCTGGCCCAGACGACCAGACTATATAAATTTGTGGGGTTGGATTTTTTGCCCCACCTCCAAACAT GGGTTTACAATGTCACCCGCGGCAAGGGCATGGGTCAGCATGCCTTCCATACTAACGCCAGGAACGCCCT CAACGTCTCTCAGGCGTGGCGTTGGTCCTTACCTTACGAAAAGGTTTCCCAGCTTCAAGATGCCTGCGGT GAGGCTATGGATTTGCTGGGATACCTCCAGGTCAGATCTCAACAAGAACAAGGCAACCTGTCCCTGGATC TTCTGTCCTCCTCCCATATCTTGGGGCAGGTCTTCCGAGAAGGTTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_011998 |
| Insert Size | 1167 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_011998.4, NP_036128.3 |
| RefSeq Size | 2000 bp |
| RefSeq ORF | 1167 bp |
| Locus ID | 26887 |
| UniProt ID | Q9R1I1 |
| Gene Summary | Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the transfer of sulfate to position 6 of non-reducing N-acetylglucosamine (GlcNAc) residues within mucin-associated glycans that ultimately serve as SELL ligands. SELL ligands are present in high endothelial cells (HEVs) and play a central role in lymphocyte homing at sites of inflammation. Participates in biosynthesis of SELL ligand sialyl 6-sulfo Lewis X on SELL counter-receptors SPN/CD43, GLYCAM1 and MADCAM1. Also involved in biosynthesis of SELL ligand recognized by MECA-79 antibody. Plays a central role in lymphocyte trafficking during chronic inflammation. Has a catalytic preference for core 2-branched mucin-type O-glycans. Can use GlcNAcbeta1-6[Galbeta1-3]GalNAc-pNP (core 2), GlcNAcbeta1-6ManOMe and GlcNAcbeta1-2Man oligosaccharide structures as acceptors. Has also activity toward core 3 of GlcNAcbeta1-3GalNAc-pNP. Its substrate specificity may be influenced by its subcellular location.[UniProtKB/Swiss-Prot Function] |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG206058 | Chst4 (tGFP-tagged) - Mouse carbohydrate (chondroitin 6/keratan) sulfotransferase 4 (Chst4) |
CNY 3,710.00 |
|
| MR206058 | Chst4 (Myc-DDK-tagged) - Mouse carbohydrate (chondroitin 6/keratan) sulfotransferase 4 (Chst4) |
CNY 3,656.00 |
|
| MR206058L3 | Lenti ORF clone of Chst4 (Myc-DDK-tagged) - Mouse carbohydrate (chondroitin 6/keratan) sulfotransferase 4 (Chst4) |
CNY 5,230.00 |
|
| MR206058L4 | Lenti ORF clone of Chst4 (mGFP-tagged) - Mouse carbohydrate (chondroitin 6/keratan) sulfotransferase 4 (Chst4) |
CNY 5,230.00 |
