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Chst4 (NM_011998) Mouse Tagged ORF Clone
CAT＃: MG206058
- TrueORF®
Chst4 (tGFP-tagged) - Mouse carbohydrate (chondroitin 6/keratan) sulfotransferase 4 (Chst4)
ORF Plasmid: DDK
"NM_011998" in other vectors (4)
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CNY 3,710.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | Gn6st-2; GST-3; HEC-GlcNAc6ST |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG206058 representing NM_011998
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATGCTGTTGAAGAAAGGGAGGCTGCTGATGTTCCTGGGTTCCCAGGTCATCGTTGTAGCTCTCTTCA TCCATATGTCCGTCCACAGACACCTTTCCCAGAGGGAGGAGTCCAGGAGGCCCGTGCATGTGCTGGTGCT GTCTTCCTGGCGGTCAGGATCCTCTTTTGTGGGACAGCTTTTCGGGCAGCACCCGGATGTGTTCTACCTG ATGGAGCCTGCCTGGCATGTGTGGATGACTTTCACCAGCAGCACAGCCTGGAAGCTGCACATGGCTGTGC GGGATCTTCTGCGTTCCGTCTTCCTGTGTGACATGAGCGTCTTTGATGCCTACATGAACCCAGGCCCCCG GAAACAGTCCAGCCTCTTCCAGTGGGAGCAAAGCCGGGCCCTGTGCTCAGCGCCTGTGTGTGACTTCTTC CCTGCCCACGAGATCAGCTCACCCAAGCACTGCAAGCTGCTCTGCGGTCAGCAGCCCTTTGATATGGTGG AGAAGGCCTGCCGCTCTCACGGCTTCGTGGTACTCAAGGAGGTGCGTTTTCTCAGCCTGCAGGCCCTCTA TCCACTGCTCACGGACCCTTCCCTCAACCTGCACGTCGTGCACCTGGTCCGAGACCCCCGGGCCGTGTTC CGATCCCGGGAGCACACCACCATAGAACTCGTGGTTGACAGTCATATTGTGCTAGGGCAGCATTTGGAAA CGATCAAGGAGGAAGACCAGCCCTATTATGCCATGAAGATCATCTGCAAAAGCCAGGTGGACATAGTCAA GGCCATCCAAACCCTCCCTGAAGCTCTGCAGCAGCGCTACCTGTTCCTGAGGTATGAGGACCTGGTTCGG GCACCCCTGGCCCAGACGACCAGACTATATAAATTTGTGGGGTTGGATTTTTTGCCCCACCTCCAAACAT GGGTTCACAATGTCACCCGCGGCAAGGGCATGGGTCAGCATGCCTTCCATACTAACGCCAGGAACGCCCT CAACGTCTCTCAGGCGTGGCGTTGGTCCTTACCTTACGAAAAGGTTTCCCAGCTTCAAGATGCCTGCGGT GAGGCTATGGATTTGCTGGGATACCTCCAGGTCAGATCTCAACAAGAACAAGGCAACCTGTCCCTGGATC TTCTGTCCTCCTCCCATATCTTGGGGCAGGTCTTCCGAGAAGGT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG206058 representing NM_011998
Red=Cloning site Green=Tags(s) MMLLKKGRLLMFLGSQVIVVALFIHMSVHRHLSQREESRRPVHVLVLSSWRSGSSFVGQLFGQHPDVFYL MEPAWHVWMTFTSSTAWKLHMAVRDLLRSVFLCDMSVFDAYMNPGPRKQSSLFQWEQSRALCSAPVCDFF PAHEISSPKHCKLLCGQQPFDMVEKACRSHGFVVLKEVRFLSLQALYPLLTDPSLNLHVVHLVRDPRAVF RSREHTTIELVVDSHIVLGQHLETIKEEDQPYYAMKIICKSQVDIVKAIQTLPEALQQRYLFLRYEDLVR APLAQTTRLYKFVGLDFLPHLQTWVHNVTRGKGMGQHAFHTNARNALNVSQAWRWSLPYEKVSQLQDACG EAMDLLGYLQVRSQQEQGNLSLDLLSSSHILGQVFREG TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_011998 |
| ORF Size | 1164 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_011998.3, NP_036128.2 |
| RefSeq Size | 2201 bp |
| RefSeq ORF | 1167 bp |
| Locus ID | 26887 |
| UniProt ID | Q9R1I1 |
| Gene Summary | Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the transfer of sulfate to position 6 of non-reducing N-acetylglucosamine (GlcNAc) residues within mucin-associated glycans that ultimately serve as SELL ligands. SELL ligands are present in high endothelial cells (HEVs) and play a central role in lymphocyte homing at sites of inflammation. Participates in biosynthesis of SELL ligand sialyl 6-sulfo Lewis X on SELL counter-receptors SPN/CD43, GLYCAM1 and MADCAM1. Also involved in biosynthesis of SELL ligand recognized by MECA-79 antibody. Plays a central role in lymphocyte trafficking during chronic inflammation. Has a catalytic preference for core 2-branched mucin-type O-glycans. Can use GlcNAcbeta1-6[Galbeta1-3]GalNAc-pNP (core 2), GlcNAcbeta1-6ManOMe and GlcNAcbeta1-2Man oligosaccharide structures as acceptors. Has also activity toward core 3 of GlcNAcbeta1-3GalNAc-pNP. Its substrate specificity may be influenced by its subcellular location.[UniProtKB/Swiss-Prot Function] |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC207463 | Chst4 (untagged) - Mouse carbohydrate (chondroitin 6/keratan) sulfotransferase 4 (Chst4), (10ug) |
CNY 3,990.00 |
|
| MR206058 | Chst4 (Myc-DDK-tagged) - Mouse carbohydrate (chondroitin 6/keratan) sulfotransferase 4 (Chst4) |
CNY 3,330.00 |
|
| MR206058L3 | Lenti ORF clone of Chst4 (Myc-DDK-tagged) - Mouse carbohydrate (chondroitin 6/keratan) sulfotransferase 4 (Chst4) |
CNY 5,230.00 |
|
| MR206058L4 | Lenti ORF clone of Chst4 (mGFP-tagged) - Mouse carbohydrate (chondroitin 6/keratan) sulfotransferase 4 (Chst4) |
CNY 5,230.00 |
