Wee1 (NM_009516) Mouse Untagged Clone

CAT#: MC205607

Wee1 (untagged) - Mouse WEE 1 homolog 1 (S. pombe) (Wee1), (10ug)



  "NM_009516" in other vectors (4)

CNY 4,816.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms Wee1A
Vector PCMV6-Kan/Neo
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>BC006852
CGGACGCGTGGGCGGACGCGTGGGGGGAAGCCGCGGAACCCGAACTTGCGGACGAACTTGCGGAGCCTTT CGCCAGCACTGGACCGGCCAGTCTCCGCTGGACATCCCGCGCCGCAGCCGTCGCCGTCGCCCCGTCGTGA GCTTGACGGGCCTCGGAGTTGCCGCCGCGCAGGCCCGCTGCAGCCTCAGCCCGGCGCGCCCGATCGCACG TGGCGGACCCGCCCCCTGTGCCAGCCGCGACCTGGACGCCGCCGGCCTCTGCTCGCCGGTCCTGTGTTCT CCCGTGCTCCGCCGTGACTGCAGCCGGGAAATGAGCTTCCTGAGCCGACAGCAGCCGCCGCCCACCCGCC GCGTAGGAGCCGCTTACAGTCTGCGGCAGAAGCTCATCTTCTCGCCGGGCAGCGACTGTGAGGAAGAGGA GGAGGAGGAGGAGGAAGGCAGCGGCCACAGTACGGGGGAGGACTCGGCCTTCCAGGAACCGGATTCGCCC CTGCCTTCTGCACGCAGCCCTGCCGAGGCCGAGGCCGAGCGCCGCCGTCGCTCGCCCGGCGCGGAGCCCA GCAGCCCGGGAGAGCTGGAGGACGACTTGCTGCTGCAGGGCGGCGGCGGAGGCGCGCAAGCGGCGGGTGG AGGCGCCGAGGGCGACTCTTGGGAGGAGGAGGGTTTCGGCTCCTCGTCGCCCGTCAAATCACCTTCGACC GCCTACTTTCTGAGCAGCCCGTTCTCGCCAGTGCGCTGCGGCGGCCCAGGGGATGCCTCCCCGCAGGGTT GCGGGGCTCCGCGAGCCATGGACGACCCCTGCTCGCCGCAGCCCGACTACCCGAGCACCCCTCCGCACAA GACCTTCCGCAAGCTGCGCCTGTTCGACACGCCGCACACTCCCAAGAGTTTGCTTTCCAAAGCGCGAGTT ATTGATTCCGGCTCTGTTAAACTCCGGGGTAGTTCTCTATTCATGGACACAGAAAAGTCAGGAAAAAGAG AATTCGATACACGGCAAACTCCCCAAGTGAATATTAACCCCTTTACTCCGGATCCTGTACTGCTCCATTC CTCAGGACGGTGTCGTGGGAGAAAGAGAGCGTATTTTAATGATTCCTCTGAAGACATGGAAGCCAGTGAT TATGAGTTTGAAGATGAAACAAGACCTGCCAAAAGAATTACAATTACTGAAAGCAACATGAAGTCACGGT ATACAACTGAATTTCATGAGCTGGAGAAAATTGGTTCTGGAGAATTTGGTTCTGTGTTTAAATGTGTGAA GAGGTTAGATGGATGCATTTATGCCATTAAACGATCAAAAAAACCATTGGCTGGCTCTGTTGATGAGCAG AATGCTTTGAGAGAAGTGTATGCTCACGCTGTGCTTGGACAGCACCCCCACGTCGTTCGCTATTTCTCTG CCTGGGCAGAGGATGACCACATGCTTATACAGAACGAATACTGTAATGGTGGGAGTTTAGCTGATGCTAT AAGTGAGAACTACAGAGTCATGAGCTACTTGACTGAAGTAGAGCTGAAGGATCTCCTTTTGCAAGTTGGC CGGGGCTTGAGATACATACATTCAATGTCTTTGGTTCACATGGATATAAAACCTAGTAATATTTTTATAT CTCGAACCTCAATCCCAAATGCTGTCTCTGAGGAAGGAGATGAAGATGACTGGATATCCAATAAAGTTAT GTTTAAAATAGGTGATCTTGGGCATGTAACAAGAATCTCTAGTCCTCAAGTTGAAGAAGGTGATAGTCGT TTTCTAGCAAATGAAGTTTTACAAGAGAACTATAGCCATCTACCGAAAGCAGATATTTTTGCTCTTGCTC TCACAGTCGTATGTGCTGCTGGTGCTGAACCCCTTCCCAGAAATGGAGAGCAGTGGCACGAGATCCGGCA GGGTCGGTTACCTCGGATCCCACAAGTGCTTTCCCAGGAGGTGACAGAGTTGCTGAGAGTCATGATTCAT CCTGACCCAGAGAGAAGGCCTTCAGCAATGGAGCTGGTGAAGCATTCAGTGTTGTTGTCTGCATCTAGAA AGAGCGCAGAGCAGTTACGAATAGAATTGAATGCTGAAAAATTCAAAAATTCTCTTTTGCAGAAAGAACT CAAGAAAGCCCAAATGGCAGCCAAAGTTGCAGCTGAGGAACGGGCACTCTTCACAGATCGGATGGCCACT AGATCCACCACCCAGAGTAATAGAACTTCTCGACTTATTGGAAAGAAAATGAACCGCTCTGTCAGCCTTA CTATATACTGAACTGCTCACATTCCCCAGCCCTTCCCCACTCAAAGGAAAAAACTGTGACAAGAGGAAGC TAGGTTGGAATCACTGCTAGAGCCCACTTCGTGATTACTTGCCCATTGGTATCAGTAGTTTTCCTAAAAC GTAACGCCTTCTGTGACTTTGAGAATTACAGTTGAGAGCTGTATTCTGACTTGGCTCTGTCAGGCTTTTG TCTCATCTCCCGTCTGTTTCATGTAGGATGTGGGTCACTGTTGGATGTTCCAGCCCCCTTTGCGGGGTGC GCCACTGTGGTGGTGTGCTGCTTCTCATTCACTGTTGCAGTGTGGTGAAAGGTGTCTTTCCTGGTTGTGA CCTCTAGAAAGGACTCAAGGGCTTTACTGCGAACATCCTCTCCCTTCAAAAAGGGAAATGCTCAGAGACT CAGTACTCCTCTGCCTCCAATGTACCTGTGTCAGTCTTGCGTTTCTTTTTTCTATGTGAATTACACTTGT ATATCCAACTGGGAGCACTTTAGGCATCGTGTGCGCCATGGGATGGTCATTCTGTGGAGGCCTTGCCTTG TGAATTTGCTGCTATTTTAGTTTCCTCTTGCTGTAAAACTAGCATTAAACAATCATTGTTGTTAATAGGC TTTCTTTCAGAACAATTATGTGAAATATATAGCTGCTCTTCATGAAAAGCAGCTTCGCATCTTTTTCTTT GGACTTTGAAGCTACTGCATTGAAAAAGTGCACCTACCCCTCCCTGTTTGGATGCTGTATTAATGTAGTA TAATTATTACTGGTTTTGTAAATTTTTCTTATAATGTCCCCTGTCTTTAAAACATGTTTCCTTCCTCCTG TCAAGTCTTGTACTTGGTTGTATGGTTAGCCTATTTTTAAATGTTTTGCCCAGTTTCTCTTCAATATTTG TGTATATAAACTGATCTTGGTAATACTGTACATAGCTGTTTAAAAATACCAGAATGACTTCTGACTATTC CAAGTTTTCCACAAAATACATTTTACCTGTGAATAGCCATTTGACTAACAATATTTGGCTAGTAGATGCT GCCCTCCAAAAGGAGGGAAATGTCTCACTTGTTTTCCTATTAACTTTGGTCTGAAACACATTTGCACTTG TCTGTTTGACTTGTGTCTTATTAACATTGTCTGGTGTATTAAAAGTCATTCCGTGCTTACCTTAAAAAAA AAAAAAAA
Restriction Sites RsrII-NotI     
ACCN NM_009516
Insert Size 1941 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC006852, AAH06852
RefSeq Size 3438 bp
RefSeq ORF 1941 bp
Locus ID 22390
UniProt ID P47810
Gene Summary Acts as a negative regulator of entry into mitosis (G2 to M transition) by protecting the nucleus from cytoplasmically activated cyclin B1-complexed CDK1 before the onset of mitosis by mediating phosphorylation of CDK1 on 'Tyr-15'. Specifically phosphorylates and inactivates cyclin B1-complexed CDK1 reaching a maximum during G2 phase and a minimum as cells enter M phase. Phosphorylation of cyclin B1-CDK1 occurs exclusively on 'Tyr-15' and phosphorylation of monomeric CDK1 does not occur. Its activity increases during S and G2 phases and decreases at M phase when it is hyperphosphorylated. A correlated decrease in protein level occurs at M/G1 phase, probably due to its degradation (By similarity).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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