Pkmyt1 (NM_023058) Mouse Untagged Clone
CAT#: MC203172
Pkmyt1 (untagged) - Mouse protein kinase, membrane associated tyrosine/threonine 1 (Pkmyt1), (10ug)
CNY 3,656.00
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Specifications
Product Data | |
Type | Mouse Untagged Clone |
Tag | Tag Free |
Synonyms | 6230424P17; AW209059; Myt1 |
Vector | PCMV6-Kan/Neo |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>BC025061
CGCTGCTGCGCCACGCCCAGCCTCCCCAGGTACTTGTGGCCCACACGACCTTCATGTGGACAATACAACG TGTGTGTGGCAAAGAAGTGGCTCTCTTGTGGGCCACAGGACCAAGTGCTGTTTGAGTGGACACATTGCTT TGTGCTCGGATCCTTTAGCGTTGGCCATGACCATGCCCACCGAGGGCACCCCACCACCCCTAAGTGGTAC CCCCATCCCAGTTCCAGCTTACTTCCGACACGCAGAGCCTGGTTTCTCCCTCAAAAGGCCTGGGGGCCTC AGTCGGAGCCTCCCACCTCGGCCCCCTGCCAAGGGCTGCATCCCTGTCAGCCGTCTATTCCCCCCTCGCA CCCCAGGCTGGCACCAGCCCCAGCCCCGGAGGGTGTCTTTCCTGTGTGAGACCTCAGAGCCCCTGCAGAG TCCTGGGTATGACCCGAGCCGGCCCGAGTCCTTCTTTCAGCAGAACTTCCAGAGGCTCAGCCGCCTGGGT CATGGCTCATATGGAGAAGTCTTCAAGGTGCGCTCTAAGGAAGATGGGCGACTCTATGCTGTTAAGCGCT ACATGTCGCCATTCCGCGGCCCCAAAGACCGAACTCGTAAACTGGCTGAGGTAGGTGGCCATGAGAAAGT GGGGCAGCATCCACACTGCGTGAGACTGGAGCGGGCCTGGGAGGAGGGTGGCATCCTATACCTGCAGACA GAACTCTGCGGGCCCAGCCTGCAGCAACACTGTGAAGCCTGGGGGGCCAGCCTGCCAGAGGCCCAGGTCT GGGGCTACTTGCGGGACATTCTTCTGGCTCTGGACCATCTACATAGTCAAGGCCTAGTTCACCTTGATGT CAAGCCTGCCAACATCTTCTTGGGTCCCCGGGGCCGCTGCAAGCTGGGCGACTTTGGACTACTGGTGGAG CTGGGTTCAACCGGTGCTGGCGAGGCCCAGGAGGGAGATCCTCGCTACATGGCCCCAGAACTGCTGCAGG GCTCTTATGGGACAGCAGCAGATGTGTTCAGTCTGGGTCTCACCATCTTGGAAGTGGCCTGTAACATGGA ACTGCCCCATGGTGGGGAGGGCTGGCAGCAGCTGCGCCAGGGATACTTGCCCCCTGAGTTCACTGCTGGT CTGTCTTCTGAGCTGCGTTCTGTCCTCGCCATGATGCTGGAGCCTGACCCCCAGCTTCGAGCCACAGCTG AGGCCCTGTTGGCCTTACCCATGCTGAGGCAGCCACGTCCCTGGAATGTTCTGTGGTATATGGCTGCAGA AGCCCTAAGTCGAGGCTGGGCCCTGTGGCAGGCCCTGGTCACTCTGCTCTGCTGGCTCTGGCACGGGCTG GTGCATCCTGCCAGTTGGCTGCAGCCTCCAGGCCCGCCGGCCACACCACCTGGCTCTCCACCTTGCAGCC CCCTCCTGGACAGCACCCTCTCCAGCAGCTGGGATAATGACAGCATAGGTCCCTCACTCTCCCCAGAGAC CGTCCTGTCCCGGATCACTAGAAGAACCTCTACCCCTCGGGGCAGGTACATACCTAGGGATGCCCTGGAC CTAACTGATGTGGACTCAGAGCCTCCAAGAGGTCCCTGCCCCACCTTTGAGCCAAGGAACCTCCTCAGCC TGTTTGAGGACTCCCTAGACCCAGCCTGAGCACAGGCCCAGCCAGCCTTGGTGCTTTTAAACCTCTGACC GTCTTGCCCACCTCTCCCCTGAAACTTCCGTGTCTAATAAAAAGTAATGCGTCTTGGGAACACCCAAGGT TGCTCATGTGGCAAAAAAAAAAAAAAAAAAAAAAAAAA |
Restriction Sites | RsrII-NotI |
ACCN | NM_023058 |
Insert Size | 1473 bp |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | BC025061, AAH25061 |
RefSeq Size | 1788 bp |
RefSeq ORF | 1473 bp |
Locus ID | 268930 |
UniProt ID | Q9ESG9 |
Gene Summary | Acts as a negative regulator of entry into mitosis (G2 to M transition) by phosphorylation of the CDK1 kinase specifically when CDK1 is complexed to cyclins. Mediates phosphorylation of CDK1 predominantly on 'Thr-14'. Also involved in Golgi fragmentation. May be involved in phosphorylation of CDK1 on 'Tyr-15' to a lesser degree, however tyrosine kinase activity is unclear and may be indirect. May be a downstream target of Notch signaling pathway during eye development (By similarity).[UniProtKB/Swiss-Prot Function] |
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Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MG207845 | Pkmyt1 (tGFP-tagged) - Mouse protein kinase, membrane associated tyrosine/threonine 1 (Pkmyt1) |
CNY 3,710.00 |
|
MR207845 | Pkmyt1 (Myc-DDK-tagged) - Mouse protein kinase, membrane associated tyrosine/threonine 1 (Pkmyt1) |
CNY 3,656.00 |
|
MR207845L3 | Lenti ORF clone of Pkmyt1 (Myc-DDK-tagged) - Mouse protein kinase, membrane associated tyrosine/threonine 1 (Pkmyt1) |
CNY 5,230.00 |
|
MR207845L4 | Lenti ORF clone of Pkmyt1 (mGFP-tagged) - Mouse protein kinase, membrane associated tyrosine/threonine 1 (Pkmyt1) |
CNY 5,230.00 |