Pkmyt1 (NM_023058) Mouse Tagged ORF Clone
CAT#: MG207845
- TrueORF®
Pkmyt1 (tGFP-tagged) - Mouse protein kinase, membrane associated tyrosine/threonine 1 (Pkmyt1)
ORF Plasmid: DDK
"NM_023058" in other vectors (4)
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CNY 3,710.00
CNY 1,000.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | 6230424P17; AW209059; Myt1 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG207845 representing NM_023058
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACCATGCCCACCGAGGGCACCCCACCACCCCTAAGTGGTACCCCCATCCCAGTTCCAGCTTACTTCC GACACGCAGAGCCTGGTTTCTCCCTCAAAAGGCCTGGGGGCCTCAGTCGGAGCCTCCCACCTCGGCCCCC TGCCAAGGGCTGCATCCCTGTCAGCCGTCTATTCCCCCCTCGCACCCCAGGCTGGCACCAGCCCCAGCCC CGGAGGGTGTCTTTCCTGTGTGAGACCTCAGAGCCCCTGCAGAGTCCTGGGTATGACCCGAGCCGGCCCG AGTCCTTCTTTCAGCAGAACTTCCAGAGGCTCAGCCGCCTGGGTCATGGCTCATATGGAGAAGTCTTCAA GGTGCGCTCTAAGGAAGATGGGCGACTCTATGCTGTTAAGCGCTACATGTCGCCATTCCGCGGCCCCAAA GACCGAACTCGTAAACTGGCTGAGGTAGGTGGCCATGAGAAAGTGGGGCAGCATCCACACTGCGTGAGAC TGGAGCGGGCCTGGGAGGAGGGTGGCATCCTATACCTGCAGACAGAACTCTGCGGGCCCAGCCTGCAGCA ACACTGTGAAGCCTGGGGGGCCAGCCTGCCAGAGGCCCAGGTCTGGGGCTACTTGCGGGACATTCTTCTG GCTCTGGACCATCTACATAGTCAAGGCCTAGTTCACCTTGATGTCAAGCCTGCCAACATCTTCTTGGGTC CCCGGGGCCGCTGCAAGCTGGGCGACTTTGGACTACTGGTGGAGCTGGGTTCAACCGGTGCTGGCGAGGC CCAGGAGGGAGATCCTCGCTACATGGCCCCAGAACTGCTGCAGGGCTCTTATGGGACAGCAGCAGATGTG TTCAGTCTGGGTCTCACCATCTTGGAAGTGGCCTGTAACATGGAACTGCCCCATGGTGGGGAGGGCTGGC AGCAGCTGCGCCAGGGATACTTGCCCCCTGAGTTCACTGCTGGTCTGTCTTCTGAGCTGCGTTCTGTCCT CGCCATGATGCTGGAGCCTGACCCCCAGCTTCGAGCCACAGCTGAGGCCCTGTTGGCCTTACCCATGCTG AGGCAGCCACGTCCCTGGAATGTTCTGTGGTATATGGCTGCAGAAGCCCTAAGTCGAGGCTGGGCCCTGT GGCAGGCCCTGGTCACTCTGCTCTGCTGGCTCTGGCACGGGCTGGTGCATCCTGCCAGTTGGCTGCAGCC TCCAGGCCCGCCGGCCACACCACCTGGCTCTCCACCTTGCAGCCCCCTCCTGGACAGCACCCTCTCCAGC AGCTGGGATAATGACAGCATAGGTCCCTCACTCTCCCCAGAGACCGTCCTGTCCCGGATCACTAGAAGAA CCTCTACCCCTCGGGGCAGGTACATACCTAGGGATGCCCTGGACCTAACTGATGTGGACTCAGAGCCTCC AAGAGGTCCCTGCCCCACCTTTGAGCCAAGGAACCTCCTCAGCCTGTTTGAGGACTCCCTAGACCCAGCC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG207845 representing NM_023058
Red=Cloning site Green=Tags(s) MTMPTEGTPPPLSGTPIPVPAYFRHAEPGFSLKRPGGLSRSLPPRPPAKGCIPVSRLFPPRTPGWHQPQP RRVSFLCETSEPLQSPGYDPSRPESFFQQNFQRLSRLGHGSYGEVFKVRSKEDGRLYAVKRYMSPFRGPK DRTRKLAEVGGHEKVGQHPHCVRLERAWEEGGILYLQTELCGPSLQQHCEAWGASLPEAQVWGYLRDILL ALDHLHSQGLVHLDVKPANIFLGPRGRCKLGDFGLLVELGSTGAGEAQEGDPRYMAPELLQGSYGTAADV FSLGLTILEVACNMELPHGGEGWQQLRQGYLPPEFTAGLSSELRSVLAMMLEPDPQLRATAEALLALPML RQPRPWNVLWYMAAEALSRGWALWQALVTLLCWLWHGLVHPASWLQPPGPPATPPGSPPCSPLLDSTLSS SWDNDSIGPSLSPETVLSRITRRTSTPRGRYIPRDALDLTDVDSEPPRGPCPTFEPRNLLSLFEDSLDPA TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_023058 |
ORF Size | 1470 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_023058.2, NP_075545.2 |
RefSeq Size | 2040 bp |
RefSeq ORF | 1473 bp |
Locus ID | 268930 |
UniProt ID | Q9ESG9 |
Gene Summary | Acts as a negative regulator of entry into mitosis (G2 to M transition) by phosphorylation of the CDK1 kinase specifically when CDK1 is complexed to cyclins. Mediates phosphorylation of CDK1 predominantly on 'Thr-14'. Also involved in Golgi fragmentation. May be involved in phosphorylation of CDK1 on 'Tyr-15' to a lesser degree, however tyrosine kinase activity is unclear and may be indirect. May be a downstream target of Notch signaling pathway during eye development (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC203172 | Pkmyt1 (untagged) - Mouse protein kinase, membrane associated tyrosine/threonine 1 (Pkmyt1), (10ug) |
CNY 3,656.00 |
|
MR207845 | Pkmyt1 (Myc-DDK-tagged) - Mouse protein kinase, membrane associated tyrosine/threonine 1 (Pkmyt1) |
CNY 3,656.00 |
|
MR207845L3 | Lenti ORF clone of Pkmyt1 (Myc-DDK-tagged) - Mouse protein kinase, membrane associated tyrosine/threonine 1 (Pkmyt1) |
CNY 5,230.00 |
|
MR207845L4 | Lenti ORF clone of Pkmyt1 (mGFP-tagged) - Mouse protein kinase, membrane associated tyrosine/threonine 1 (Pkmyt1) |
CNY 5,230.00 |