United States
Germany
Japan
United Kingdom
China
Parn (NM_028761) Mouse Untagged Clone
CAT＃: MC203133
Parn (untagged) - Mouse poly(A)-specific ribonuclease (deadenylation nuclease) (Parn), (10ug)
CN¥ 4,656.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | 1200003I18Rik; DAN |
| Vector | PCMV6-Kan/Neo |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>BC021899
GTGCTGTTTCGTGGCGGCCTCCGACATCCCCACGCTGCTTTTGCTGCGGAACTCTGCGCGTGGACCGGCG TCAGCCTGGGCGGGGCCAAGGTTCCGTCTGCGCCGCAGGATGGAGATAATCCGGAGCAATTTTAAGATTA ATCTTCACAAAGTGTACCAGGCCATAGAGGAGGCTGACTTCTTCGCCATCGATGGGGAGTTTTCAGGAAT CAGCGATGGACCCTCAGTAACAGCATTAACAAGTGGTTTTGACACCCCAGAAGAGAGATATCAGAAGCTT AAAAAGCATTCCATGGACTTTTTGCTGTTTCAGTTTGGCCTTTGTGCTTTTAAGTATGACCACACAGATT CCAAGCATGTAACGAAGTCATTTAACTTCTATGTTTTCCCCAAGCCTTTCAGTAGGTCCTCACCAGATGT CAAGTTTGTTTGCCAGAGCTCCAGCATTGACTTCCTGGCGAGCCAAGGATTTGACTTTAATAAAGTGTTT TGCAGTGGGATTCCATATCTGAATCAGGAAGAAGAAAGGCAGCTGAGAGAGCAATTCGATGAAAAACGGT CTCAAGCCAATGGGGCAGGAGCTCTGGCAAAATGTCCTGTGACCATCCCTGAGGATCAGAAGAAGTTTAT TGACCAAGTAATAGAGAAGATAGAGGATTTCCTACAAAGTGAAGAGAAGAGGAGCCTGGAACTTGACCCA TGCACTGGGTTCCAAAGAAAACTTATTTATCAGACTTTGAGTTGGAAGTATCCTAAAGGCATTCATGTTG AGACATTAGAGACTGACAAGAAGGAAAGACATATAGTTATCAGCAAGGTGGATGAAGAAGAACGCAAAAG GAGAGAGCAGGAGAAGTATACAAAGGAACAGGAGGAGCTGAATGATGCTGTGGGGTTTTCAAGAGTCATC CATGCCATTGCTAATTCGGGGAAGCTGGTTGTGGGACACAACATGCTCTTGGATGTCATGCATACGATTC ATCAGTTCTACTGCCCCCTGCCTGCGGACCTGAATGAGTTTAAGGAGATGGCAATATGTGTCTTCCCCAG ACTCTTGGATACTAAGTTGATGGCCAGCACACAGCCTTTTAAGGATATCATTAACAACACATCCCTTGCA GAGTTGGAAAAGCGGTTGAAAGAGACACCTTTTGACCCTCCCAAAGTTGAAAGTGCAGAAGGCTTTCCAA GCTACGACACAGCTTCTGAGCAGCTTCATGAGGCGGGGTACGATGCCTACATCACAGGGCTCTGCTTCAT CTCCATGGCAAATTACTTAGGTTCTTTACTCAGTCCTCCAAAAATGTGTGTGTCTGCCAGATCAAAGCTC ATTGAACCCTTTTTTAACAAGTTATTTCTTATGAGGGTCATGGATATTCCCTATTTAAACTTGGAAGGGC CAGACTTGCAGCCTAAGCGGGACCATGTTCTCCACGTGACCTTCCCCAAAGAGTGGAAAACCAGCGACCT GTACCAGCTCTTCAGCGCCTTCGGTAACATTCAGATATCCTGGATTGATGATACATCAGCCTTCGTTTCT CTCAGCCAGCCAGAACAAGTACAAATTGCCGTTAATACCAGCAAGTACGCTGAAAGTTATCGGATCCAGA CCTATGCTGAGTATGTGGGAAAGAAGCAGGAAGGCAAGCAGGTCAAGAGGAAGTGGACAGAAGACAGTTG GAAGGAGGTGGACAGAAAGCGGCCCCACATGCAGGGCCCCTGTTACCACAGCAACAGCTTCACAGCAGCT GGCGTGCTTGGAAAGAGAACGCTGAGTCCTGACCCAAGGGAAGCTGCCTTGGAGGACAGAGAATCAGAGG AGGTATCTGACTCGGAGCTTGAACAGACAGATTCCTGTACAGACCCCCTCCCAGAGGGAAGGAAAAAGTC CAAGAAGTTAAAACGAATGAAGAAGGAGCTTTCCTTGGCAGGAAGTGTCTCGGATAGCCCTGCCGTGCTC TTTGAAGTCCCTGACACATGGTAGTGGAGTACTGCTGCGACAGCCCAGCCTACCTGCTTGGAAGCCATAC TGTAGACACTTAACCAGATATGGCCTGGGGGAGTTTTGGAACCAGATTGTTTCCTGCAAAGCTACTTTTT ACTGTTTTGGCTGCATCTATTGTCCCTTTTAGTCTAAACTGACCTTTGGCACACGTCGTGGTTGAGGGCT TCTGCAAGCGTGTGTGTTATTAACTAATTACTTCCTGCTCCTTGAAAACCTGCCTGCCCTGGTGAATGTT GGCAAGTCCACCCAGGGAGTAAGTCAGCTGTGAACCACGCCAGGAGAGCCAACCTCCGGGGTGTTCTAGG CAGATTTCTGCAGAGCTTCTCCTGCCCGAGATGTGCCCTGCCATCCTCCCACTGCTGCCCTGAAACAGCA CACAGGCTTGTCTTTTCAGTGGGAGATTCCTGTGCTAGCTCAGGCTCATCTCAACAGATGCCCAGGCTTT GAGAGCTTGTTATTTCAAGTTTCTTTCCCTCAGATAGGAGTGTGTCCCGGGTGTGGTGACATGTGTGTGA GAAGCTGGCTCTTCTGTTCCTACGCACAGGGTTCACGGTGCAGCAGCTTCTCCTGGTTTTTACTGTATGT CTTTGTGCATCTGTATCTGGGTACATGGTGTGTGTGTCTGTCTCTGTGTGTGTATGTGTCTGTGTGTGTG TCTGTCTGTCTGTCTGTGACAGTGGCAGGCCTACAGCATGCATCAAGTCCTCACTCAGACAGGTTTCAGT GGCAGAGCCTCACTTGCTCCTGCAGGATACCCCACAGCAGACACTTCTGGACATGTAACGCTTTTGTATC ATGAAAATAAAAGTGAAAAAGTAATTTCAAAAAGCTGAAATTAAAGAAAATTTCCTAGAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAA |
| Restriction Sites | RsrII-NotI |
| ACCN | NM_028761 |
| Insert Size | 1875 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC021899, AAH21899 |
| RefSeq Size | 2900 bp |
| RefSeq ORF | 1875 bp |
| Locus ID | 74108 |
| UniProt ID | Q8VDG3 |
| Gene Summary | 3'-exoribonuclease that has a preference for poly(A) tails of mRNAs, thereby efficiently degrading poly(A) tails. Exonucleolytic degradation of the poly(A) tail is often the first step in the decay of eukaryotic mRNAs and is also used to silence certain maternal mRNAs translationally during oocyte maturation and early embryonic development. Interacts with both the 3'-end poly(A) tail and the 5'-end cap structure during degradation, the interaction with the cap structure being required for an efficient degradation of poly(A) tails. Involved in nonsense-mediated mRNA decay, a critical process of selective degradation of mRNAs that contain premature stop codons. Also involved in degradation of inherently unstable mRNAs that contain AU-rich elements (AREs) in their 3' UTR, possibly via its interaction with KHSRP. Probably mediates the removal of poly(A) tails of AREs mRNAs, which constitutes the first step of destabilization (By similarity). Also able to recognize poly(A) tails of microRNAs such as MIR21 and H/ACA box snoRNAs (small nucleolar RNAs) leading to leading to microRNAs degradation or snoRNA increased stability (By similarity).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) encodes the longest isoform (1). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG209527 | Parn (tGFP-tagged) - Mouse poly(A)-specific ribonuclease (deadenylation nuclease) (Parn) |
CN¥ 6,080.00 |
|
| MR209527 | Parn (Myc-DDK-tagged) - Mouse poly(A)-specific ribonuclease (deadenylation nuclease) (Parn) |
CN¥ 4,656.00 |
|
| MR209527L3 | Lenti ORF clone of Parn (Myc-DDK-tagged) - Mouse poly(A)-specific ribonuclease (deadenylation nuclease) (Parn) |
CN¥ 7,410.00 |
|
| MR209527L4 | Lenti ORF clone of Parn (mGFP-tagged) - Mouse poly(A)-specific ribonuclease (deadenylation nuclease) (Parn) |
CN¥ 7,410.00 |
