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Mkl1 (NM_001082536) Mouse Untagged Clone
CAT＃: MC202660
Mkl1 (untagged) - Mouse MKL (megakaryoblastic leukemia)/myocardin-like 1 (Mkl1), transcript variant 2, (10ug)
CN¥ 6,808.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | AMKL; Bsac; Mal; Mrtf-A |
| Vector | PCMV6-Kan/Neo |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>BC050941 sequence for NM_001082536
CGCTCGCCACGCTCCCTCGGCTTGCGCGGACCTCTCCCTAACTTGGTGCAGTCTGACTGGTACTAGAGGC TCGGGAGGATACGTGAGCGGCTGGCACCGGAGTGGGGCGAGCCGCGGAGCCCGGGCCAGGAGGCGAACGA GGCGGTTACCATCACGAAAGCCAAGGTGGATTTCTCCAGTGTGGTGTGCCTGCCCCCTTCCGTCATTGCT GTGAATGGGCTGGACGGAGGAGGGGCTGGCGAAAATGACGACGAGCCAGTGCTCCTGTCTCTGTCTGCGG CCCCCAGCCCCCAGAGCGAAGCTGTTGCCAATGAACTGCAGGAGCTGTCCCTGCAGCCCGAGCTGACTCT AGGCCTCCATCCTGGGAGGAACCCCAATTTACCTCCACTTAGTGAGCGGAAGAATGTGCTGCAGTTGAAG CTCCAGCAGCGGCGGACCCGGGAGGAACTGGTGAGCCAAGGGATCATGCCGCCTTTGAAAAGCCCCGCTG CATTTCATGAGCAGAGAAGAAGCCTGGAGCGGGCCAGGACCGAGGACTATTTGAAACGGAAGATCCGTTC CCGGCCCGAGAGAGCAGAGCTGGTCAGGATGCACATTCTGGAAGAGACCTCGGCTGAGCCTTCGCTCCAG GCCAAGCAGCTGAAGCTGAAGAGAGCCAGGCTGGCTGATGACCTCAATGAAAAGATTGCACAGAGGCCTG GCCCCATGGAGCTGGTGGAGAAGAATATCCTGCCTGTGGAGTCCAGCCTGAAGGAGGCTATCATTGTGGG CCAGGTAAATTACCCAAAGGTAGCAGACAGTTCCTCCTTCGACGAGGACAGCAGCGATGCCCTGTCTCCT GAGCAGCCTGCCAGCCATGAGTCCCAGGGTTCAGTGCCATCACCCTTGGAGTCCCGAGTCAGTGATCCAC TGCCCAGTGCCACCTCCATATCACCCACTCAGGTTCTTTCTCAACTCCCAATGGCTCCGGATCCTGGAGA GACGCTTTTTCTGGCAGAGCAGCCTCCTCTGCCTCCCGCACCTCTGCTGCCCCCAAGCCTAGCCAATGGA AGCATCGTCCCCACTGCCAAGCCTGCTCCCACACTCATCAAGCAAAGCCAACCCAAGTCTGCCAGCGAGA AATCACAGCGCAGCAAGAAGGCCAAGGAGCTGAAGCCAAAGGTGAAGAAGCTCAAGTACCACCAGTACAT CCCCCCGGACCAGAAGCAGGACAAGGGGGCGCCCGCCATGGACTCCTCCTATGCCAAGATCCTGCAGCAG CAGCAGCTCTTCCTGCAGCTGCAGATCCTCAACCAGCAGCAGCAGCAGCAGCAGCAACAGCACTACAACT ACCAGGCCATCCTGCCTGCCCCTCCCAAGCCCTCGGCTGAGACTCCTGGAAGCAGTGCCCCTACCCCATC ACGCAGCCTCTCCACCAGTAGCAGCCCCAGCTCAGGCACCCCAGGGCCCAGCGGGCTGGCACGCCAGAGC AGCACCGCACTAGCTGCCAAACCAGGAGCCCTGCCAGCCAACCTGGATGACATGAAGGTGGCAGAGCTGA AGCAAGAACTGAAGTTGCGGTCCCTTCCCGTCTCAGGCACCAAGACAGAGCTGATAGAACGCCTGCGTGC CTACCAAGACCAAGTCAGCCCAGCTCCAGGAGCCCCCAAGGCCCCTGCCACCACCTCTGTGCTGTCCAAG GCTGGTGAGGTAGTGGTCGCCTTCCCTGCGGCCCTGCTAAGCACAGGGTCAGCTCTTGTAACAGCAGGCC TTGCACCAGCTGAGATGGTGGTGGCCACAGTAACCAGCAATGGCATGGTGAAGTTTGGCAGCACAGGCTC CACACCCCCCGTGTCTCCCACCCCTTCAGAGCGCTCACTGCTCAGCACGGGTGATGAGAATTCCACACCT GGGGATGCCTTTGGTGAAATGGTGACATCGCCGCTGACACAGCTCACCCTGCAGGCCTCCCCACTGCAGA TCGTGAAGGAGGAGGGTGCCCGTGCTGCGTCCTGCTGTCTAAGCCCTGGTGCTCGGGCTGAGCTGGAGGG ACTGGACAAGGACCAGATGCTGCAGGAGAAGGACAAGCAGATTGAGGAGCTGACCCGAATGCTCCAACAG AAGCAGCAGCTGGTTGAGCTGCTGCGGCTACAGCTGGAGCAGCAGAAGCGGGCCCAGCAGCCAGCCCCAG CCAGCAGCCCTGTGAAGAGGGAAAGTGGTTTCTCCAGTTGCCAGCTGAGCTGCCAGCCCCAGGGCTCTGC CCATGCTTTTGGCTCTGGCCTAGTGGTTCCCACTACCAACCATGGAGACACTCAGGCCCCAGCGCCAGAG TCCCCACCTGTGGTGGTGAAGCAGGAAGCTGGGCCACCTGAGCCAGATCTGGCCCCCAGCTCCCAGCTGC TCTTGGGCTCCCAGGGCACCAGCTTCCTCAAGAGGGTCAGCCCTCCTACCCTGGTCACTGACTCTACAGG GACTCACCTCATCCTCACTGTGACCAATAAGAGTGCTGATGGCCCTGGCTTGCCTGCAGGGAGCCCCCAG CAGCCCTTGTCCCAGCCTGGTTCTCCAGCCCCTGGTCCACCTGCCCAGATGGACCTGGAGCACCCACCTC AGCCTCCGTTTGCAACCCCCACATCTCTGCTGAAGAAGGAGCCCCCTGGTTATGAAGAGACTGTGACCCA GCAGCCTAAGCAGCAGGAAAATGGCTCCTCCAGTCAGCACATGGATGATCTGTTTGATATTCTTATTCAG AGTGGAGAGATTTCAGCAGATTTCAAAGAGCCACCATCCCTACCAGGCAAGGAAAAGTCACCTCCAGCAG CAGCAGCGTATGGGCCTCCATTGACACCACAACCCTCGCCTTTGAGTGAACTCCCCCAAGCTGCTCCTCC ACCAGGTTCCCCCACCCTCCCAGGGCGCCTTGAAGACTTCCTGGAGAGCAGCACAGGGCTGCCCCTGCTG ACAAGTGGGCACGAGGGACCAGAACCCCTTTCCCTCATTGATGACCTCCACAGCCAGATGCTGAGCAGCT CCGCCATCCTGGACCACCCCCCATCACCCATGGACACCTCTGAATTGCACTTTGCTCCTGAGCCCAGCAG TGGTATGGGCCTGGACCTGGCTGTTGGCCACCTGGACAGCATGGACTGGCTGGAGCTGTCGTCTGGTGGC CCTGTGCTCAGCCTGGCTCCCCTCAGCACTGCAGCCCCCAGCCTCTTCTCGATGGACTTCCTGGATGGAC ACGACTTGCAGCTCCACTGGGATTCCTGCTTGTAGCCCTCTGACTCCAGACATGCGCTAGGGAAGGGTCA GAGGTTGGGGAGCCACACAACCATACCCAGTGTACGCTCCTGTGACTGTGAGCCTCGACAATCACAGTCC CTAGGGGCTGCAGCAGAAACCCTATACTTGGCTCAGGCTCACTGTGGGCAGTGGGCATAAGCTGTCAAGA AACAAATCTGGGCCCATTCTGAGGTTTTGGGAGGCTGGCCAGAGCCAGTTCAATTCGACCTCCTTTTCTG AGGTCAGAGGTGCACATCTGGGTGAAGTTTTTTGCCTAGGTGGGCTTATAGTCAACCCGCCTTCCAGGTC CTTCACTGCAAGGAAGAAACTAGCCAGGCCTCTACTCACCCATTCCCTATTTTAGCCAGTCCCAGGACCC GGCACTATGCCATTTCAGTCTTGGTGTATAGTATAACCACTGGTGGCAAAAGTCTGTATAGATGTATATA CCTCTATATTATTATATATCAACTTACATATATACATATATTTTTAGGGGTGGTGGGAAATGGGTGTGGT TACCTCCACATGCTTTCACAACGTAAAAAGCCTGGAGGGCTGTTTGGGGCTTGATACAGGATCATTGTGA GAAGTGTGCTATATGACCCAGTATCCAACCTGGCATGCAGCTGCCCAGGCCCACCCCACACATGTATGTA ACATGTAGACACCTGCCATTGCCTCCTACACGCCACCCTGCCCTGCTTCCTGGCTCCTGAGTGCCACAAG GGCAGAGGTGCCTCCTGTCCTCCGCCAGCAACTCTCCACTCACCCACATTCCGTCCCACCTCCCCACTGC ATCCACCCTGGCCTGGGAGTGGGAGTGGGGTGGGTCCCAATGCATCTGCTGGGGGCTGAGAGGTACCTTC TGCCCATCACCAGCCTTGTTCTGTGCTGACTCAATGCAGAGAAGGGATAGAGCCATCAAAAGCAGTGCTG GGGCAAAGGCGGAAAGCTGTGTGAACTTTTTAAAATAAAACAAAAACACTGAAAAAAAAAAAAAAAAAA |
| Restriction Sites | AscI-NotI |
| ACCN | NM_001082536 |
| Insert Size | 2790 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC050941, AAH50941 |
| RefSeq Size | 4269 bp |
| RefSeq ORF | 2790 bp |
| Locus ID | 223701 |
| UniProt ID | Q8K4J6 |
| Gene Summary | Transcription coactivator that associates with the serum response factor (SRF) transcription factor to control expression of genes regulating the cytoskeleton during development, morphogenesis and cell migration (PubMed:12019265, PubMed:12732141, PubMed:17588931, PubMed:19350017, PubMed:24732378). The SRF-MRTFA complex activity responds to Rho GTPase-induced changes in cellular globular actin (G-actin) concentration, thereby coupling cytoskeletal gene expression to cytoskeletal dynamics (PubMed:24732378). MRTFA binds G-actin via its RPEL repeats, regulating activity of the MRTFA-SRF complex (PubMed:12732141, PubMed:17588931). Activity is also regulated by filamentous actin (F-actin) in the nucleus (PubMed:23558171, PubMed:25759381).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) differs in the 5' UTR and coding sequence compared to variant 1. The encoded isoform (2) has a longer and distinct N-terminus compared to isoform 1. This variant uses a non-AUG translation start codon. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG211216 | Mkl1 (tGFP-tagged) - Mouse MKL (megakaryoblastic leukemia)/myocardin-like 1 (Mkl1), transcript variant 2 |
CN¥ 7,890.00 |
|
| MR211216 | Mkl1 (Myc-DDK-tagged) - Mouse MKL (megakaryoblastic leukemia)/myocardin-like 1 (Mkl1), transcript variant 2 |
CN¥ 6,808.00 |
|
| MR211216L3 | Lenti ORF clone of Mkl1 (Myc-DDK-tagged) - Mouse MKL (megakaryoblastic leukemia)/myocardin-like 1 (Mkl1), transcript variant 2 |
CN¥ 9,030.00 |
|
| MR211216L4 | Lenti ORF clone of Mkl1 (mGFP-tagged) - Mouse MKL (megakaryoblastic leukemia)/myocardin-like 1 (Mkl1), transcript variant 2 |
CN¥ 9,030.00 |
