Ciao1 (NM_025296) Mouse Untagged Clone

CAT#: MC200380

Ciao1 (untagged) - Mouse cytosolic iron-sulfur protein assembly 1 homolog (S. cerevisiae) (Ciao1), (10ug)



  "NM_025296" in other vectors (4)

CNY 3,656.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (4)
Rabbit Polyclonal Anti-Ciao1 Antibody
    • 50 ug

CNY 4,628.00


TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms AW210570; Wdr39
Vector PCMV6-Kan/Neo
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>BC004089 sequence for NM_025296
CCCACGCGTCCGATTTTGCAGAAGCTGTACATCTGACGGTCTCAAGGTTCTATAGTGCCCAGCGCGTTTG GCGCGTTGCTTCTCTCGGTGTGCTCTCCTTACCCTAAAACAGTCGCCATGAAAGATTCTCTGGTACTGCA GAGCCGCGTCCCGGCGCACCCGGATTCCCGCTGCTGGTTCCTGGCCTGGAACCCCTCGGGGACCCTGTTG GCTTCCTGCGGAGGCGACCGTAAAATTCGCATCTGGGGCACCGAGGGTGACAGCTGGATTTGCAAGTCTG TCCTTTCTGAAGGCCATCAGCGCACCGTTAGGAAAGTGGCCTGGTCCCCGTGTGGAAATTACCTGGCATC TGCCAGCTTTGATGCCACCACTTGTATTTGGAAGAAGAACCAGGATGACTTTGAGTGTGTGACCACTCTT GAGGGCCATGAAAATGAGGTCAAGTCAGTGGCTTGGGCCCCATCTGGCAACCTCTTGGCTACCTGCAGCA GAGATAAGAGCGTGTGGGTCTGGGAAGTTGATGAAGAGGATGAGTATGAGTGTGTCAGTGTCCTCAGCTC TCATACGCAGGATGTTAAGCACGTGGTTTGGCACCCGAGCCAGGAGCTTTTAGCTTCTGCTAGCTATGAT GACACAGTGAAGCTATACCAGGAAGAAGGGGATGACTGGGTCTGCTGTGCCACCCTCGAAGGTCATGAGT CCACTGTTTGGAGCATAGCCTTTGATCCCAGCGGCCAGCGGCTGGCATCTTGCAGTGATGACCGAACTGT ACGCATCTGGCGCCAGTATCTACCGGGCAATGAACAAGGGGTGGCATGCAGTGGCTCTGACCCCAGCTGG AAATGTATCTGCACTTTGTCGGGCTTCCACACCAGGACCATTTACGACGTTGCTTGGTGTCAGCTGACAG GGGCCTTGGCTACAGCTTGTGGAGATGATGCCATCCGAGTGTTTGAGGAAGACCCAGGCTCAGACCCACA GCAGCCCACCTTCTCTCTGACAGCCCATCTTCGTCAGGCCCATTCCCAGGATGTCAACTGTGTGGCTTGG AATCCCAAGGAGCCAGGGCTCCTGGCCTCTTGTAGTGATGATGGGGAGGTAGCCTTCTGGGAGTATCACC AGCCTGCAGGTCTCTGAGCTATAGCTTGACTTGGGAGTCTTGATTCCCACAGAAAACGTCCTAAGACTTC TAGTCGTCAAGAAGACCTGAAGCATCCTTGACCTTCATTTTGCTCAGGACTATTCACTGTTTCCCTTCCT TGATTATGAGGAAGCCATTGGAGAGAGCCACAGAACAGCAGTATATTGTATTAAAGTTTTGCTTGATTTA GGGCATGGTGTTACATTTTGGGGGTAAAATATATTTATAATCAATATATATGAAAAAAAAAAAAAAA
Restriction Sites RsrII-NotI     
ACCN NM_025296
Insert Size 1020 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC004089, AAH04089
RefSeq Size 1397 bp
RefSeq ORF 1020 bp
Locus ID 26371
UniProt ID Q99KN2
Gene Summary Key component of the cytosolic iron-sulfur protein assembly (CIA) complex, a multiprotein complex that mediates the incorporation of iron-sulfur cluster into extramitochondrial Fe/S proteins (By similarity). As a CIA complex component, interacts specifically with CIAO2A or CIAO2B and MMS19 to assist different branches of iron-sulfur protein assembly, depending of its interactors. The complex CIAO1:CIAO2B:MMS19 binds to and facilitates the assembly of most cytosolic-nuclear Fe/S proteins. CIAO1:CIAO2A specifically matures ACO1 and stabilizes IREB2 (By similarity). Seems to specifically modulate the transactivation activity of WT1. As part of the mitotic spindle-associated MMXD complex it may play a role in chromosome segregation (By similarity).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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