Chicken IgG / Chicken IgY (H+L chain) Goat Polyclonal Antibody

Specifications

Product Data
Product Name	Chicken IgG / Chicken IgY (H+L chain) Goat Polyclonal Antibody
Recommend Dilution	Suitable for immunoblotting, ELISA, immunohistochemistry, immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency. This product has been assayed against 1.0 ug of Chicken IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:20,000 to 1:80,000 of the reconstitution concentration is suggested for this product.
Reactivity	Chicken
Host	Goat
Immunogen	Chicken IgG whole molecule.
Formulation	0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 contaning 0.01% (w/v) Sodium Azide as preservative and 10 mg/ml Bovine Serum Albumin (BSA, IgG and Protease free) as stabilizer. Label: Biotin State: Lyophilized purified IgG fraction. Label: Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC) Molar radio: 10-20 BAC molecules per Goat IgG molecule
Concentration	2.0 mg/ml (before lyophil. by UV absorbance at 280 nm)
Purification	Immunoaffinity chromatography.
Conjugation	Biotin
Storage Condition	Store vial at 2-8°C prior to restoration. Restore with 1.0 ml of deionized water (or equivalent). Centrifuge product if not completely clear after standing at room temperature. This product is stable for one month at 2-8°C as an undiluted liquid. For extended storage reconstitute product with 50% glycerol instead of water and then aliquot contents and freeze at -20°C or below. Dilute only prior to immediate use. Avoid cycles of freezing and thawing.

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.