Rat IgG (F(ab)2 Fragment) Fc specific Goat Polyclonal Antibody

Specifications

Product Data
Product Name	Rat IgG (F(ab)2 Fragment) Fc specific Goat Polyclonal Antibody
Recommend Dilution	Suiatble for use Immunoblotting, ELISA, Immunohistochemistry, Immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. Recommended Dilution(s): This product has been assayed against 1.0 µg of Rat IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:15,000 to 1:90,000 of the reconstitution concentration is suggested for this product.
Reactivity	Rat
Host	Goat
Immunogen	Rat IgG F(c) fragment
Formulation	0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 10 mg/ml Bovine Serum Albumin (BSA) IgG and Protease free as stabilizer and 0.01% (w/v) Sodium Azide as preservative. Label: Biotin State: Lyophilized F(ab')2 fragments. Label: N-Hydroxysuccinimide Ester (BAC) Molar radio: 10-20 BAC molecules per Goat IgG molecule.
Concentration	lot specific
Purification	Immunoaffinity chromatography.
Conjugation	Biotin
Storage Condition	Store vial at 2-8°C prior to restoration. Restore with 1.0 ml of deionized water (or equivalent). For extended storage add glycerol to 50% and then aliquot contents and freeze at -20°C or below. Centrifuge product if not completely clear after standing at room temperature. This product is stable for one month at 2-8°C as an undiluted liquid. Dilute only prior to immediate use. Avoid repeated freezing and thawing.

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