Rat IgG (F(ab)2 Fragment) Fc specific Goat Polyclonal Antibody

Specifications

Product Data
Product Name	Rat IgG (F(ab)2 Fragment) Fc specific Goat Polyclonal Antibody
Recommend Dilution	Suitable for Immunoblotting (Western, or dot blot, 1:20,000-1:200,000), ELISA (1:20,000-1:200,000), Immunoperoxidase electron microscopy and Immunohistochemistry (1:500-1:5,000) as well as other peroxidase-antibody based enzymatic assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. Recommended Dilution(s): This product has been assayed against 1.0 ug of Rat IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature.
Reactivity	Rat
Host	Goat
Immunogen	Rat IgG F(c) whole molecule.
Formulation	0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 10 mg/ml Bovine Serum Albumin (BSA, IgG and Protease free) as stabilizer and 0.01% (w/v) Gentamicin Sulfate as preservative. Label: HRP State: Lyophilized F(ab')2 fragments. Label: Horseradish Peroxidase
Concentration	lot specific
Purification	Immunoaffinity chromatography.
Conjugation	HRP
Storage Condition	Store vial at 2-8°C prior to restoration. Restore with 0.5 ml of deionized water (or equivalent). For extended storage add glycerol to 50% and then aliquot contents and freeze at -20°C or below. Centrifuge product if not completely clear after standing at room temperature. This product is stable for one month at 2-8°C as an undiluted liquid. Dilute only prior to immediate use. Avoid repeated freezing and thawing.

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.