Human IgG (F(ab)2 specific) Rabbit Polyclonal Antibody

Specifications

Product Data
Product Name	Human IgG (F(ab)2 specific) Rabbit Polyclonal Antibody
Recommend Dilution	Suitable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.  Recommended Dilutions:  ELISA: 1/10,000-1/50,000. Western Blot: 1/1,000-1/10,000. Immunohistochemistry: 1/500-2,500. This product has been assayed against 1.0 ug of Human IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1/20,000 to 1/100,000 of the reconstitution concentration is suggested for this product.
Reactivity	Human
Host	Rabbit
Immunogen	Human IgG F(ab')2 fragment
Formulation	0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 Label: HRP State: Lyophilized Ig fraction Stabilizer: 10 mg/ml BSA (IgG and Protease free) Preservative: 0.01% (w/v) Gentamicin Sulfate Label: Horseradish Peroxidase
Concentration	2.0 mg/ml (by UV absorbance at 280 nm)
Purification	Immunoaffinity chromatography
Conjugation	HRP
Storage Condition	Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month  or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.