Human IgG (F(ab)2 specific) Rabbit Polyclonal Antibody

Specifications

Product Data
Product Name	Human IgG (F(ab)2 specific) Rabbit Polyclonal Antibody
Recommend Dilution	Suitable for Immunoblotting, ELISA, Immunohistochemistry, Immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency. Recommended Dilutions: This product has been assayed against 1.0 µg of Human IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1/30,000 to 1/120,000 of the reconstitution concentration is suggested for this product.
Reactivity	Human
Host	Rabbit
Immunogen	Human IgG F(ab')2 fragment.
Formulation	0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.4, with 10 mg/ml BSA (IgG and Protease free) as stabilizer and 0.01% (w/v) Thimerisol as preservative. Label: Biotin State: Lyophilized purified Ig fraction. Label: Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC) Molar radio: 10-20 BAC molecules per Goat IgG molecule.
Concentration	2.0 mg/ml (by UV absorbance at 280 nm)
Purification	Prepared from monospecific antiserum by Immunoaffinity Chromatography using Human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
Conjugation	Biotin
Storage Condition	Store vial at 2-8°C prior to restoration. For extended storage add glycerol to 50% and then aliquot contents and freeze at -20°C or below. Centrifuge product if not completely clear after standing at room temperature. This antibody is stable for one month at 2-8°C as an undiluted liquid. Dilute only prior to immediate use. Avoid repeated freezing and thawing.

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.