Human IgG (F(ab)2 specific) Goat Polyclonal Antibody

Specifications

Product Data
Product Name	Human IgG (F(ab)2 specific) Goat Polyclonal Antibody
Applications	ELISA, ID, IF, IHC
Recommend Dilution	Direct staining of fixed cell and tissue substrates, to demonstrate the intracellular presence of free of Ig bound subunits of both kappa and lambda type. The absence of the Fc domain in the conjugate ensures minimal interaction with the tissue components and cell surfaces other than the primary antibody activity. This conjugate is primarily intended for use in cell surface membrane staining procedures, to identify and quantitate Ig on B cells, especially if interference by Fc activity is expected. Recommended Dilutions: 1/20-1/80. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Reactivity	Human
Host	Goat
Immunogen	Purified Fab prepared from normal IgG isolated from pooled Human serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Formulation	PBS, pH 7.2 without presrevatives. Label: FITC State: Lyophilized Fab fragments. Label: Fluorescein Isothiocyanate Absorption emission: 492 nm / 515 nm Molar radio: Fluorochrome/IgG = ~1.8
Concentration	4.7 mg/ml
Purification	Hyperimmune antisera with strong precipitating activity are selected for fractionation by salt-precipitation and purification of the IgG fraction by DEAE-chromatography.
Conjugation	FITC
Storage Condition	Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.