Human IgG (F(ab)2 specific) Goat Polyclonal Antibody

Specifications

Product Data
Product Name	Human IgG (F(ab)2 specific) Goat Polyclonal Antibody
Applications	ELISA, ID, IF, IHC
Recommend Dilution	Direct staining of fixed cell and tissue substrates, to demonstrate the intracellular presence of free of Ig bound subunits of both kappa and lambda type. The absence of the Fc domain in the conjugate ensures minimal interaction with the tissue components and cell surfaces other than the primary antibody activity. This conjugate is primarily intended for use in cell surface membrane staining procedures, to identify and quantitate Ig on B cells, especially if interference by Fc activity is expected. Recommended Dilutions: 1/10-1/40 This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Reactivity	Human
Host	Goat
Immunogen	Purified Fab prepared from normal IgG isolated from pooled Human serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Formulation	PBS, pH 7.2 without presrevatives. Label: TRITC State: Lyophilized Fab fragments. Label: Tetramethylrhodamine Isothiocyanate Absorption emission: 554 nm / 573 nm Molar radio: Fluorochrome/IgG = ~2.2
Concentration	10 mg/ml
Purification	Hyperimmune antisera with strong precipitating activity are selected for fractionation by salt-precipitation and purification of the IgG fraction by DEAE-chromatography.
Conjugation	TRITC
Storage Condition	Lyophilized conjugate can be shipped at ambient temperature and may be stored at 2-8°C or at -20°C for prolonged storage. Reconstituted can be stored (into small aliquots) at -20°C. Avoid Repeated thawing and freezing. This product is photosensitive and should be protected from light.
Note	Fluorescent marker: Tetramethylrhodamine isothiocyanate isomer R. It has an orange-red fluorescence. To avoid nonspecific background staining, specially synthesized and exceptionally pure crystalline isomer R has been used instead of the usual racemic mixture. Although its fluorescence efficiency is less than of FITC, TRITC conjugates have the advantage of significantly less photo bleaching. This facilitates their use in quantitative cell-counting procedures.

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