H3FA (HIST1H3A) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	IF, IP
Recommend Dilution	WB 0.1-1 µg/ml ELISA 0.01-0.1 µg/ml IP 2-5 µg/ml IHC 2-10 µg/ml FC 5-10 µg/ml
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Immunogen	A synthetic peptide containing AR[Me2-K]ST in which Me2-K corresponds to Dimethyl lysine 27 of human histone H3.
Formulation	This affinity purified antibody is supplied in sterile Phosphate buffered saline (pH7.2) containing antibody stabilizer.
Purification	The Rabbit IgG is purified by site-modified Epitope Affinity Purification.
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	17 kDa
Gene Name	histone cluster 1, H3a
Database Link	NP_003520 Entrez Gene 360198 MouseEntrez Gene 679994 RatEntrez Gene 8350 Human UniProt ID P68431
Background	Histones are basic nuclear proteins that together with DNA make up the nucleosome structure in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The methylation of specific residues in the histone tails is a central modification for regulatingepigenetic transitions in chromatin. Whereas methylation of histone H3 on lysine 4, 36, and 79 has been linked with gene activation, methylation of H3 on lysines 9, 27 and histone H4 on lysine 20 is associated with heterochromatin and some repressed genes with euchromatin. Modified lysine residues can exist in a mono-, di-, or tri-methylated state, while the arginine residues can be mono- or di-methylated. Histone H3 Lys4 trimethylation (H3-K4me3) is a conserved mark of actively transcribed chromatin. This antibody is specific for histone H3 tri-methylated at K27. The sequence is found in all mammals and a wide range of species, including D. melanogaster, Arabidopsis, Chicken and Xenopus. The antibody will react with any of the above species where the trimethylation modification is present.
Synonyms	A; H3; H3FA
Reference Data
Protein Pathways	Systemic lupus erythematosus

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