GRP78 (HSPA5) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	FC, ICC/IF, IHC, Simple Western, WB
Recommend Dilution	Flow Cytometry: 1:150, Immunocytochemistry/ Immunofluorescence: 1:50, Immunohistochemistry-Paraffin, Immunohistochemistry Free-Floating, Western Blot: 0.5 ug/ml, Simple Western: 1:25, Immunohistochemistry: 1:200
Reactivity	Human, Mouse, Rat, Chicken, Sheep
Host	Rabbit
Clonality	Polyclonal
Immunogen	Synthetic peptide made to an internal portion of human GRP78 (within residues 250-300). [Swiss-Prot# P11021]
Formulation	PBS, 0.05% Sodium Azide. Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Concentration	lot specific
Purification	Immunogen affinity purified
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	78 kDa
Gene Name	heat shock protein family A (Hsp70) member 5
Database Link	NP_005338 Entrez Gene 14828 MouseEntrez Gene 25617 RatEntrez Gene 3309 Human UniProt ID P11021
Background	HSP70 family member GRP78 (78 kD glucose-regulated protein) is localized in ER lumen as well as melanosomes (stage I to stage IV) where it facilitates protein folding/assembly, protein quality control, Ca2+ binding, and regulates ER stress signaling. GRP78 interacts with DNAJC1 and is a component of EIF2 complex (CELF1/CUGBP1, CALR, CALR3, EIF2S1, EIF2S2, HSP90B1 and HSPA5) as well as chaperone multiprotein complex (DNAJB11, HSP90B1, HSPA5, HYOU, PDIA2, PDIA4, PDIA6, PPIB, SDF2L1, UGT1A1, ERP29). GRP78 also interacts with TMEM132A as well as TRIM21, and may form a complex with ERLEC1, OS9, SEL1L and SYVN1. In cancerous cells, GRP78 overexpression is associated with UPR activation, including upregulation of associated proteins such as PERK, ATF6, CHOP etc that further regulate JNK and NF-kB untimately leading to survival, tumor progression, angiogenesis, resistance to therapy and metastasis. GRP78 is a signaling receptor for activated alpha2-macroglobulin, plasminogen kringle 5, and microplasminogen, and plays role in viral entry of coxsackie B as well as dengue fever viruses. GRP78 is also involved in regulation of tissue factor procoagulant activity and functions as a receptor for angiogenic peptides via VEGFRs independent mechanism. Cell surface GRP78 is found associated with diverse proteins including VDAC, MHC-I, Cripto and DnaJ-like protein MTJ-1. Because GRP78 is present on the surface of cancer cells but not on normal cells in vivo, it provide an exciting opportunity for cancer targeting.
Synonyms	BIP; GRP78; HEL-S-89n; MIF2
Note	This GRP78 antibody is useful for immunocytochemistry/immunofluorescence and western blot where a band is seen at ~78 kDa. For information on the flow cytometry application, see the publication PMID: 20208072. Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID 24089213)
Reference Data
Protein Families	Druggable Genome
Protein Pathways	Antigen processing and presentation, Prion diseases

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