Neurofibromin (NF1) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ICC/IF, IHC, WB
Recommend Dilution	Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin: 1:200-1:500, Immunohistochemistry-Frozen: 1:200-1:500, Immunohistochemistry: 1:200-1:500, Western Blot: 1:500-1:2000
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Immunogen	An N-terminal peptide of Neurofibromin 1 (within amino acids 1-50). [UniProt# P21359]
Formulation	Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Concentration	lot specific
Purification	Whole antisera
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Gene Name	neurofibromin 1
Database Link	NP_001121619 Entrez Gene 18015 MouseEntrez Gene 24592 RatEntrez Gene 4763 Human UniProt ID P21359
Background	Neurofibromin 1 (NF1), the Nf1 gene product, is a large protein which contains a central Ras-GTPase-activating (RasGAP) domain and is suggested to function as a negative regulator of proto-oncogene RAS and downstream effectors. It stimulates the GTPase activity of RAS and acts as regulator of RAS signaling. Consistent with its biochemical activity, NF1 functions as a tumor-suppressor gene in juvenile myelomonocytic leukemia (JMML), and diseased BM cells exhibit aberrant Raf/MEK/ERK signaling. Moreover, NF1 shows greater affinity for Ras GAP, but lower specific activity. Besides JMML, defects in NF1 have also been linked to neurofibromatosis type 1 (NF1), Watson syndrome (WS), familial spinal neurofibromatosis (FSNF), neurofibromatosis-Noonan syndrome (NFNS), colorectal cancer (CRC).
Synonyms	NFNS; VRNF; WSS
Note	This Neurofibromin 1 antibody can be used for Western blot and Immunohistochemistry of frozen and paraffin-embedded sections. In WB, a band-set at 220-240 kDa and a higher MW band at 450 kDa (uncharacterized) can be seen. For IHC, antigen retrieval is not required for immunostaining of frozen sections. Antigen retrieval with trypsin or citrate improves immunostaining of paraffin sections. Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID: 23910086)
Reference Data
Protein Families	Druggable Genome
Protein Pathways	MAPK signaling pathway

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