c-Myc (MYC) Mouse Monoclonal Antibody [Clone ID: 9E11]

Specifications

Product Data
Clone Name	9E11
Applications	CyTOF-ready, ELISA, FC, ICC/IF, IHC, IP, WB
Recommend Dilution	Flow (Intracellular), Chromatin Immunoprecipitation (ChIP): 2 ug/ 500 ug extract, ELISA: 1:100-1:2000, Flow Cytometry: 1:200-1:400, Immunohistochemistry: 1:100, Immunoprecipitation: 2ug/mg lysate, Western Blot: 1:500-1:1000, Immunohistochemistry-Paraffin: 1:100, Immunocytochemistry/ Immunofluorescence, CyTOF-ready, Immunohistochemistry-Frozen: 1:100
Reactivity	Human, Mouse, Chicken, Yeast
Host	Mouse
Clonality	Monoclonal
Immunogen	A synthetic peptide corresponding to amino acids 408-420 (AEEQKLISEEDL) of human c-Myc, conjugated to KLH. [UniProt# P01106]
Formulation	PBS containing 0.05% BSA, 0.05% Sodium Azide. Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Concentration	lot specific
Purification	Protein A purified
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Gene Name	v-myc avian myelocytomatosis viral oncogene homolog
Database Link	NP_002458 Entrez Gene 17869 MouseEntrez Gene 4609 Human UniProt ID P01106
Background	Myc genes are a family of proto-oncogenes (L- Myc, N- Myc and C- Myc) that codes for Myc proteins which are transcriptor factors implicated in cellular proliferation, differentiation, apoptosis, metabolism, adhesion and self-renovation of tumor stem cells. Myc protein can act as transcriptional activator/repressor, and is activated via response to diverse mitogenic signals (including Wnt, Shh and EGF) and has been found to be up-regulated in several types of cancers. c-Myc participates gene transcription regulation and binds DNA in a non-specific manner, yet can specifically recognizes core sequence 5'-CAC[GA]TG-3' also. c-Myc heterodimerization with another bHLH protein namely Myc-associated factor X (MAX) is required for efficient c-Myc- DNA binding. c-Myc interacts with several proteins such as TAF1C, SPAG9, PARP10, KDM5A, KDM5B, NO66, PIM2 and with FBXW7 when phosphorylated at Thr-58/Ser-62. c-Myc activate the transcription of growth-related genes and c- Myc overexpression induce cell-cycle progression thereby implicating in a variety of cancers. Moreover, a chromosomal aberration involving c-Myc has been linked to a form of B-cell chronic lymphocytic leukemia and defective c-MYC is responsible for Burkitt lymphoma also.
Synonyms	bHLHe39; c-Myc; MRTL; MYCC
Note	This c-Myc antibody (clone 9E11) is useful for Flow Cytometry, ChIP, Immunoprecipitation, ELISA, Immunohistochemistry- Frozen and Paraffin and Western blot.
Reference Data
Protein Families	Druggable Genome, Embryonic stem cells, Induced pluripotent stem cells, Stem cell - Pluripotency, Stem cell relevant signaling - JAK/STAT signaling pathway, Stem cell relevant signaling - TGFb/BMP signaling pathway, Stem cell relevant signaling - Wnt Signaling pathway, Transcription Factors
Protein Pathways	Acute myeloid leukemia, Bladder cancer, Cell cycle, Chronic myeloid leukemia, Colorectal cancer, Endometrial cancer, ErbB signaling pathway, Jak-STAT signaling pathway, MAPK signaling pathway, Pathways in cancer, Small cell lung cancer, TGF-beta signaling pathway, Thyroid cancer, Wnt signaling pathway

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