5 Lipoxygenase (ALOX5) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	WB
Recommend Dilution	Western Blot: 1:500-1:1000, Simple Western: 10 ug/ml
Reactivity	Human, Mouse, Rat, Bovine, Rabbit
Host	Rabbit
Clonality	Polyclonal
Immunogen	A synthetic peptide made to a C-terminal region within residues 600-674 of human 5-Lipoxygenase. [Swiss-Prot# P09917]
Formulation	PBS, 30% glycerol, 0.1% Sodium Azide. Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Concentration	lot specific
Purification	Immunogen affinity purified
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	80 kDa
Gene Name	arachidonate 5-lipoxygenase
Database Link	NP_000689 Entrez Gene 11689 MouseEntrez Gene 25290 RatEntrez Gene 240 Human UniProt ID P09917
Background	5-lipoxygenase (5LO) is a pro-inflammatory enzyme widely expressed in CNS where its level increases in an age-dependent manner and its activity is regulated by the presence/availability of FLAP (5LO activating protein) that form a functional complex with 5LO to catalyze arachidonic acid oxidation to produce lipid molecules with complex signaling properties such as leukotrienes. Reaction catalyzed by 5LO is considered as first step in the leukotriene biosynthesis and it is highly critical to inflammatory processes. Moreover, knockout of 5LO as well as FLAP has been shown to result in anxiety-like behavior in mice and 5LO is emerging as a new active player in Alzheimer's disease (AD) pathogenesis. 5LO levels are significantly increased in AD postmortem brain tissues and its genetic absence or pharmacological blockade both result in a significant reduction in the abeta levels as well as deposition in a transgenic mouse model of AD-like amyloidosis (i.e., Tg2576) mice.
Synonyms	5-LO; 5-LOX; 5LPG; LOG5
Note	This 5 Lipoxygenase antibody is useful for Western Blot, where a band is seen at ~80 kDa. There may be some non-specific bands at non-interfering lower molecular weights.
Reference Data
Protein Families	Druggable Genome
Protein Pathways	Arachidonic acid metabolism, Metabolic pathways

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