CD45 Mouse Monoclonal Antibody [Clone ID: IH-1]

Specifications

Product Data
Clone Name	IH-1
Applications	FC, IHC, IP
Recommend Dilution	Flow Cytometry. Immunohistochemistry on frozen sections. Immunoprecipitation: Immunoprecipitates proteins of M.Wt. 220,000, 210,000 and 195,000 and 180,000 from lymph node lymphocytes and proteins of M.Wt 195,000, 180,000 and 170,000 from thymocytes.
Reactivity	Guinea Pig
Host	Mouse
Clonality	Monoclonal
Immunogen	Spleen cells from immunised BALB/c mice were fused with cells of the NS1 mouse myeloma cell line.
Specificity	This antibody reacts with CD45 on all leucocytes.
Formulation	TRIS buffered saline, pH 8.0 containing 0.09% Sodium Azide State: Purified State: Liquid purified IgG
Concentration	lot specific
Purification	Affinity chromatography on Protein A
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Background	CD45 is a family of single chain transmembraneous glycoproteins consisting of at least four isoforms (220, 205, 190, 180 kDa) which share a common large intracellular domain. Their extracellular domains are heavily glycosylated. The different isoforms are produced by alternative messenger RNA splicing of three exons of a single gene on chromosome 1. CD45 is expressed on cells of the human hematopoietic lineage (including hematopoietic stem cells) with the exception of mature red cells. It is not detected on differentiated cells of other tissues. It is likely that CD45 plays an important role in signal transduction, inhibition or upregulation of various immunological functions. Antibodies recognising a common epitope on all of the isoforms are termed CD45 whilst those recognising only individual isoforms are termed CD45RA or CD45RO etc.
Synonyms	Leukocyte common antigen, L-CA, PTPRC, T200, CD45RB, CD45RO, CD45RA, CD45R
Reference Data

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