CD45 Mouse Monoclonal Antibody [Clone ID: IH-1]

Specifications

Product Data
Clone Name	IH-1
Applications	FC
Recommend Dilution	Flow Cytometry: Use 10 µl of neat-1/10 diluted antibody to label 106 cells in 100 µl.
Reactivity	Guinea Pig
Host	Mouse
Clonality	Monoclonal
Immunogen	Guinea pig leukemic B lymphocyte L2C cells
Specificity	The antibody reacts with CD45 on all leucocytes.
Formulation	PBS, pH 8.0 Label: FITC State: Liquid purified IgG fraction Stabilizer: 1% BSA Preservative: 0.09% Sodium Azide Label: Fluorescein Isothiocyanate Isomer 1
Concentration	lot specific
Purification	Affinity Chromatography on Protein A
Conjugation	FITC
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing.
Background	CD45 is a family of single chain transmembraneous glycoproteins consisting of at least four isoforms (220, 205, 190, 180 kDa) which share a common large intracellular domain. Their extracellular domains are heavily glycosylated. The different isoforms are produced by alternative messenger RNA splicing of three exons of a single gene on chromosome 1. CD45 is expressed on cells of the human hematopoietic lineage (including hematopoietic stem cells) with the exception of mature red cells. It is not detected on differentiated cells of other tissues. It is likely that CD45 plays an important role in signal transduction, inhibition or upregulation of various immunological functions. Antibodies recognising a common epitope on all of the isoforms are termed CD45 whilst those recognising only individual isoforms are termed CD45RA or CD45RO etc.
Synonyms	Leukocyte common antigen, L-CA, PTPRC, T200, CD45RB, CD45RO, CD45RA, CD45R
Note	Immunoprecipitates proteins of M.Wt. 220,000, 210,000, 195,000 and 180,000 from lymph node lymphocytes and proteins of M.Wt 195,000, 180,000 and 170,000 from thymocytes.
Reference Data

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