FITC Rat Monoclonal Antibody [Clone ID: LO-FLUO-1]

Specifications

Product Data
Clone Name	LO-FLUO-1
Applications	FC, IHC
Recommend Dilution	Flow cytometry. ELISA: 2.5 µg/ml.
Host	Rat
Clonality	Monoclonal
Immunogen	FITC conjugated mouse immunoglobulin. Spleen cells from immunised LOU/c rats were fused with cells of the IR983F rat myeloma cell line.
Specificity	This antibody binds to both free FITC and FITC conjugated to protein such as antibodies. The reagent may be useful for amplification of staining using FITC conjugated reagents.
Formulation	PBS, pH7.4 containing 0.09% Sodium Azide State: Purified State: Liquid purified IgM
Concentration	lot specific
Purification	Affinity chromatography
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Background	FITC (fluorescein isothiocyanate) is a fluorochrome dye that absorbs ultraviolet or blue light causing molecules to become excited and emit a visible yellow-green light. This emission ceases upon removal of the light causing the excitation. Fluorochrome labeling provides rapid, accurate localization of antigen-antibody interaction when one of the reactants is part of a cell, tissue or other biological structure. FITC is a commonly used marker for antibodies in immunofluorescent techniques since the conjugation of FITC to proteins is relatively easy and does not, in general, destroy the biological activity of the labeled protein. FITC is widely used as a hapten to label different proteins.
Synonyms	Fluorescein Isothiocyanate
Reference Data

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