Lipoamide Dehydrogenase (DLD) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, WB
Recommend Dilution	Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoprecipitation and most immunological methods requiring high titer and specificity. Recommended Diutions: This product has been assayed against 1.0 ug of Lipoamide Dehydrogenase [Porcine Heart] in a standard sandwich ELISA using Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) (R1364HRP) and (ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:6,000 to 1:25,000 of the reconstitution concentration is suggested for this product.
Reactivity	Porcine
Host	Rabbit
Clonality	Polyclonal
Immunogen	Lipoamide Dehydrogenase [Porcine Heart].
Specificity	This product was prepared from monospecific antiserum by a delipidation and defibrination. Assay by immunoelectrophoresis resulted in a single precipitin arc against purified and partially purified Lipoamide Dehydrogenase [Porcine Heart]. Cross reactivity against Lipoamide Dehydrogenase from other sources is unknown.
Formulation	0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01% (w/v) sodium azide as preservative. State: Serum State: Lyophilized purified Ig fraction.
Reconstitution Method	Restore with 2.0 ml of deionized water (or equivalent).
Concentration	lot specific
Purification	Multistep process.
Conjugation	Unconjugated
Storage Condition	Store vial at 2-8°C prior to restoration. For extended storage add glycerol to 50% and then aliquot contents and freeze at -20°C or below. Centrifuge product if not completely clear after standing at room temperature. This antibody is stable for one month at 2-8°C as an undiluted liquid. Dilute only prior to immediate use. Avoid repeated freezing and thawing.
Gene Name	dihydrolipoamide dehydrogenase
Database Link	UniProt ID P09622
Background	Lipoamide dehydrogenase or dihydrolipoamide dehydrogenase (DLD) is a component of the glycine cleavage system as well as of the alpha ketoacid dehydrogenase complexes. It is a homodimer. It belongs to the class I pyridine nucleotide disulfide oxidoreductase family. Defects in DLD are a cause of congenital infantile lactic acidosis. Moreover, defects in DLD are the cause of DLD deficiency; which results in extensive metabolic disturbances, including lactic acidemia, Krebs cycle dysfunction, and impaired branched amino acid degradation. DLD deficiency also causes neurological degeneration due to the sensitivity of the central nervous system to defects in oxidative metabolism.
Synonyms	DLD, GCSL, LAD, PHE3, EC 1.8.1.4, Dihydrolipoamide dehydrogenase, Glycine cleavage system L protein
Reference Data

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