Galectin 3 (LGALS3) Mouse Monoclonal Antibody [Clone ID: OTI1A4]

Specifications

Product Data
Clone Name	OTI1A4
Applications	IF, WB
Recommend Dilution	WB 1:400 - 1:2000, IF 1:100
Reactivity	Human, Mouse
Host	Mouse
Clonality	Monoclonal
Immunogen	Full length human recombinant protein of human LGALS3(NP_002297) produced in HEK293T cell.
Formulation	Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
Reconstitution Method	For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
Purification	Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	26 kDa
Gene Name	galectin 3
Database Link	NP_002297 Entrez Gene 16854 MouseEntrez Gene 3958 Human UniProt ID P17931
Background	This gene encodes a member of the galectin family of carbohydrate binding proteins. Members of this protein family have an affinity for beta-galactosides. The encoded protein is characterized by an N-terminal proline-rich tandem repeat domain and a single C-terminal carbohydrate recognition domain. This protein can self-associate through the N-terminal domain allowing it to bind to multivalent saccharide ligands. This protein localizes to the extracellular matrix, the cytoplasm and the nucleus. This protein plays a role in numerous cellular functions including apoptosis, innate immunity, cell adhesion and T-cell regulation. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Apr 2010]
Synonyms	CBP35; GAL3; GALBP; GALIG; L31; LGALS2; MAC2
Reference Data
Protein Families	Secreted Protein

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