PNPO Mouse Monoclonal Antibody [Clone ID: OTI3B3]

Specifications

Product Data
Clone Name	OTI3B3
Applications	IF, WB
Recommend Dilution	WB 1:500~2000, IF 1:100
Reactivity	Human, Dog, Rat, Monkey, Mouse
Host	Mouse
Clonality	Monoclonal
Immunogen	Full length human recombinant protein of human PNPO(NP_060599) produced in HEK293T cell.
Formulation	Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
Reconstitution Method	For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
Purification	Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	29.8 kDa
Gene Name	pyridoxamine 5'-phosphate oxidase
Database Link	NP_060599 Entrez Gene 64533 RatEntrez Gene 103711 MouseEntrez Gene 480540 DogEntrez Gene 694949 MonkeyEntrez Gene 55163 Human UniProt ID Q9NVS9
Background	The enzyme encoded by this gene catalyzes the terminal, rate-limiting step in the synthesis of pyridoxal 5'-phosphate, also known as vitamin B6. Vitamin B6 is a required co-factor for enzymes involved in both homocysteine metabolism and synthesis of neurotransmitters such as catecholamine. Mutations in this gene result in pyridoxamine 5'-phosphate oxidase (PNPO) deficiency, a form of neonatal epileptic encephalopathy. [provided by RefSeq, Oct
Synonyms	HEL-S-302; PDXPO
Reference Data
Protein Pathways	Metabolic pathways, Vitamin B6 metabolism

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