CD5 Mouse Monoclonal Antibody [Clone ID: OTI6F7]

Specifications

Product Data
Clone Name	OTI6F7
Applications	FC, IHC, WB
Recommend Dilution	WB 1:500~2000, FLOW 1:100
Reactivity	Human, Monkey
Host	Mouse
Clonality	Monoclonal
Immunogen	Full length human recombinant protein of human CD5 (NP_055022) produced in HEK293T cell.
Formulation	Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
Reconstitution Method	For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
Purification	Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	54.4 kDa
Gene Name	CD5 molecule
Database Link	NP_055022 Entrez Gene 695249 MonkeyEntrez Gene 921 Human UniProt ID P06127
Background	CD5 (Lymphocyte antigen T1/Leu-1) is a transmembrane glycoprotein which has been implicated as a receptor in the regulation of T-cell proliferation (1). CD5 is part of the scavenger receptor cysteine-rich (SRCR) family group B and it is found on 95% of thymocytes and 72% of peripheral blood lymphocytes. In lymph nodes, the main reactivity is observed in T cell areas. CD5 is expressed by T cell leukemia, lymphomas, and activated T cells. Occasionally, CD5 antigen is also expressed on a subset of B cells. Mantle cell lymphomas (same as diffuse centrocytic lymphomas) are CD5+ while the follicle center cell lymphomas are CD5. A ligand for CD72/LYB-2, CD5 has been linked to negative regulation of T cell and B cell receptors signaling (2).
Synonyms	LEU1; T1
Reference Data
Protein Families	Druggable Genome, Transmembrane
Protein Pathways	Hematopoietic cell lineage

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