MHC Class II (I-A k,b,d,q,r) Rat Monoclonal Antibody [Clone ID: ER-TR3]

Specifications

Product Data
Clone Name	ER-TR3
Applications	FC, IHC
Recommend Dilution	Flow Cytometry: 10 µg/ml (Use 10 µl of 1/10 diluted antibody to label 10e6 cells). Suggested Positive Control: mouse spleen. Has beed described to work in FACS.
Reactivity	Mouse
Host	Rat
Clonality	Monoclonal
Immunogen	Murine thymic reticulum.
Specificity	This antibody is specific for cells expressing MHC class II antigens. Antigen, epitope: MHC Class II antigens are heterodimers consisting of one alpha-chain (31-34 kD) and one beta-chain (26-29 kD). Antigen Distribution: Isolated Cells: The antigen is found on dendritic cells, B-cells and macrophages. The level of antigen detected by ER-TR1, ER-TR 2 and ER-TR 3 differs from strain to strain (see table below). Tissue Sections: The antigen is found on B-cells, interdigitating cells and macrophages in peripheral lymphoid organs but is absent from T-cells. It is also expressed as a fine reticular pattern on stromal thymic cells of the cortex and as a confluent pattern on stromal thymic cells of the medulla. Monoclonal antibody ER-TR 3 is one member of a family of monoclonal antibodies (ER-TR 3, ER-TR 2, ER-TR 1) which detect MHC class II antigens encoded by the murine Ia region of the H-2 complex, corresponding to the human HLA-DR region. They are valuable tools for studying T helper cell interaction with class II positive antigen presenting cells (dendritic cells, B-cells, macrophages). These antibodies also offer new possibilities for studying the development of T helper cells since they also stain stromal cells in the thymus.
Formulation	Phosphate buffered saline pH 7.2 (PBS) containing 10 mg/ml BSA as a stabilizer and 0.09% Sodium Azide as a preservative Label: FITC State: Liquid purified Ig fraction.
Concentration	0.1 mg/ml
Purification	Affinity Chromatography.
Conjugation	FITC
Storage Condition	Store the antibody undiluted at 2-8°C.
Background	MHC Class II antigens are heterodimers consisting of one alpha chain (31-34kD) and one beta chain (26-29kD). The family of monoclonal antibodies (ER-TR 3, ER-TR 2, ER-TR 1) detect MHC class II antigens encoded by the murine Ia region of the H-2 complex, corresponding to the human HLA-DR region. MHC Class II antigens are a valuable tool for studying T helper cell interaction with class II positive antigen presenting cells (dendritic cells, B cells, macrophages) and offer new possibilities for studying the development of T helper cells since these antibodies also stain stromal cells in the thymus. MHC Class II antigens are also inducible on a number of other cells (endothelium and epithelial cells) by interferon gamma.
Reference Data

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