MHC Class II (I-A k,b,d,q,r) Rat Monoclonal Antibody [Clone ID: ER-TR3]

Specifications

Product Data
Clone Name	ER-TR3
Applications	FC, IHC
Recommend Dilution	Immunohistochemistry on Frozen Sections: 1.5 µg/ml (1/200). Suggested Positive Control: Mouse spleen. Does not react on routinely processed paraffin sections. Has been reported to work in Flow Cytometry.
Reactivity	Mouse
Host	Rat
Clonality	Monoclonal
Immunogen	Murine thymic reticulum.
Specificity	Monoclonal antibody ER-TR3 detects MHC class II antigens encoded by the murine Ia region of the H-2 complex, corresponding to the human HLA-DR region. it is a valuable tool for studying T-helper cell interaction with class II positive antigen presenting cells (dendritic cells, B-cells, macrophages). This antibody also offers new possibilities for studying the development of T-helper cells since it also stains stromal cells in the thymus. Antigen Distribution Isolated Cells: The antigen is found on dendritic cells, B-cells and macrophages. Tissue sections: The antigen is found on B-cells, interdigitating cells and macrophages in peripheral lymphoid organs but is absent from T-cells. It is also expressed as a fine reticular pattern on stromal thymic cells of the cortex and as a confluent pattern on stromal thymic cells of the medulla.
Formulation	Stock Solution contains PBS, pH 7.2 with 5 mg/ml BSA as a stabilizer and 0.09% Sodium Azide as preservative State: Purified State: Lyophilized purified Ig fraction
Reconstitution Method	Restore with 0.5 ml distilled water.
Concentration	0.3 mg/ml
Purification	Affinity Chromatography
Conjugation	Unconjugated
Storage Condition	Store lyophilized at 2-8°C and reconstituted at -20°C. Avoid repeated freezing and thawing.
Background	MHC Class II antigens are heterodimers consisting of one alpha chain (31-34kD) and one beta chain (26-29kD). The family of monoclonal antibodies (ER-TR3, ER-TR2, ER-TR1) detect MHC class II antigens encoded by the murine Ia region of the H-2 complex, corresponding to the Human HLA-DR region. MHC Class II antigens are a valuable tool for studying T helper cell interaction with class II positive antigen presenting cells (dendritic cells, B cells, macrophages) and offer new possibilities for studying the development of T helper cells since these antibodies also stain stromal cells in the thymus. MHC Class II antigens are also inducible on a number of other cells (endothelium and epithelial cells) by interferon gamma.
Note	Protocol: Protocol with Frozen, ice-cold Acetone-Fixed Sections: The whole procedure is performed at room temperature 1. Wash in PBS 2. Block endogenous peroxidase 3. Wash in PBS 4. Block with 10 % normal goat serum in PBS for 30 min. in a humid chamber 5. Incubate with primary antibody (dilution see datasheet) for 1h in a humid chamber 6. Wash in PBS 7. Incubate with secondary antibody (peroxidase-conjugated goat anti rat IgG (H+L) minimal-cross reaction to mouse) for 1h in a humid chamber 8. Wash in PBS 9. Incubate with AEC substrate (3-amino-9-ethylcarbazol) for 12 min. 10. Wash in PBS 11. Counterstain with Mayer's hemalum
Reference Data

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