Endothelial Cells Rat Monoclonal Antibody [Clone ID: 1F10]

Specifications

Product Data
Clone Name	1F10
Applications	IHC
Recommend Dilution	Immunohistochemistry on Frozen sections: 2 µg/ml (1:200). Positive control: Human placenta. Does not react on routinely processed paraffin sections.
Reactivity	Human
Host	Rat
Clonality	Monoclonal
Immunogen	Cultured HUVECs
Specificity	This antibody recognizes an antigen on human continuous blood endothelial cells which are fully differentiated. The antibody staining is restricted to endothelial cells and does not sta in any other cell in tissues or in peripheral blood. On sinusoidal endothelial cells of the liver or lymphatic organs the antigen is inconsistently expressed or absent.
Formulation	Phosphate buffered saline pH 7.2 Label: Biotin State: Lyophilized affinity purified Ig fraction Stabilizer: 10 mg/ml bovine serum albumin Preservative: 0.01% thimerosal
Reconstitution Method	Reconstitute by adding 0.5 ml distilled water.
Conjugation	Biotin
Storage Condition	Store lyophilized at 2-8°C. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.
Background	The antigen is found in variable numbers on freshly isolated umbilical vein endotheli al cells (HUVECs). Antigen expression on cultured HUVECs is dependent on the culture conditions. Best results were obtained (40 - 70% pos.) by daily renewal of FCS containing medium supplemented with endothelial cell conditioned supernatant whereas commercia lly available endothelial cell media apparently have no influence on the antigen expression.
Synonyms	Endothelial Cell marker
Reference Data

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