Endothelial Cells Rat Monoclonal Antibody [Clone ID: 1F10]

Specifications

Product Data
Clone Name	1F10
Applications	IHC
Recommend Dilution	Immunohistochemistry on Frozen sections: 2 µg/ml (1:100); Suggested positive control: Human placenta. Antigen Distribution: The antigen is found in variable amounts on freshly isolated isolated HUVE cells. Antigen expression on cultured HUVEC is dependent on the culture conditions. Best results were obtained (40-70% pos.) by daily renewal of FCS containing medium supplemented with endothelial cell conditioned supernatant whereas commercial available specific endothelial cell media have no influence on the antigen expression (see Table 1).
Reactivity	Human
Host	Rat
Clonality	Monoclonal
Immunogen	Cultured HUVECs.
Specificity	The monoclonal antibody 1F10 recognizes an antigen on human continuous blood endothelial cells, which are fully differentiated. The antibody staining is restricted to endothelial cells and does not stain any other cell in tissues or in peripheral blood. On sinusoidal endothelial cells of the liver or lymphatic organs the antigen is inconsistently expressed or absent. The antigen is an SDS sensitive 250kD surface protein as determined by gel filtration. Specific to human continuous endothelial cells, does not react with mouse antigen, other species unknown.
Formulation	PBS, pH 7.2 containing 10 mg/ml bovine serum albumin (BSA) as a stabilizer and 0.01% thimerosal as a preservative. State: Purified State: Lyophilized purified Ig fraction.
Reconstitution Method	Restore with 0.5 ml distilled water.
Concentration	0.2 mg/ml
Purification	Affinity A chromatography.
Conjugation	Unconjugated
Storage Condition	Store the antibody at 2-8°C for one month or (in aliquots) at -20°C for longer. Do not freeze working dilutions Avoid repeated freezing and thawing.
Synonyms	Endothelial Cell marker
Reference Data

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