CD14 Mouse Monoclonal Antibody [Clone ID: X-8]

Specifications

Product Data
Clone Name	X-8
Applications	FC, IHC
Recommend Dilution	Suitable for Immunohistochemistry (IHC) on frozen sections (0.25-0.5 µg/ml, 1/800-1/1600) and FACS. Suggested positive control: Human placenta. Antigen Distribution on Isolated cells and Tissue sections: Monocytes, granulocytes, macrophages in myeloid leukaemia. Cell Lines: HL-60 without stimulation negative HL-60 stimulated with PMA 15% positive HL-60 stimulated with DMSO 15% positive U937 negative X-8 is positive on Langerhans cells, follicular dendritic cells, histiocytes and high endothelial cell venules. Functional assays.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Human monocytes
Specificity	Monoclonal antibody X-8 prevents the Lipopolysaccharide Binding Protein (LBP) independent activation of cells via soluble CD14 by blocking the binding of CD14.
Formulation	PBS, pH 7.2 with 5 mg/ml bovine serum albumin (BSA) as a stabilizer without preservatives. Label: Biotin State: Lyophilized purified Ig fraction.
Reconstitution Method	Restore with 0.5 ml distilled water.
Concentration	0.4 mg/ml
Purification	Affinity chromatography.
Conjugation	Biotin
Storage Condition	Store the antibody at 2-8°C for one month or (in aliquots) at -20°C for longer. Do not freeze working dilutions Avoid repeated freezing and thawing.
Gene Name	CD14 molecule
Database Link	Entrez Gene 929 Human UniProt ID P08571
Background	CD14 is a 53'000 - 55'000 Mr glycoprotein strongly expressed on the surface of monocytes and weakly on the surface of granulocytes. It is anchored to the surface by linkage to glycosyl phosphatidylinositol (GPI). CD14 functions as a receptor for endotoxin and is the major receptor involved in the lethal response to both endotoxin and Gram-negative bacteria. In addition to membrane CD14, soluble forms of CD14 can be detected in serum and tissue culture supernatants of cells transfected with CD14.
Synonyms	CD14
Reference Data

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