CD14 Mouse Monoclonal Antibody [Clone ID: X-8]

Specifications

Product Data
Clone Name	X-8
Applications	FC, IHC
Recommend Dilution	Immunohistochemistry on Frozen Sections: 2-4 μg/ml (1/50-1/100). Does not react on routinely processed Paraffin Sections. Recommended Positive Control: Human placenta. Has been described to work in FACS (post fixation is recommended).
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Human Monocytes.
Specificity	Monoclonal antibody X-8 prevents the Lipopolysaccharide Binding Protein (LBP) independent activation of cells via soluble CD14 by blocking the binding of CD14. BM4034 is specific for Human CD14 on monocytes, some populations of macrophages and granulocytes. The epitope has not been further characterized. Antigen Distribution Isolated Cells: Monocytes, granulocytes, myeloid leukaemia and myeloid cell lines. Tissue Sections: Positive on histiocytes, Langerhans cells, follicular dendritic cells and high endothelial venules.
Formulation	PBS pH 7.2 containing 10 mg/ml BSA as stabilizer and 0.01% Thimerosal as preservative State: Purified State: Lyophilized purified IgG fraction
Reconstitution Method	Restore by adding 0.5 ml distilled water (= Stock solution).
Concentration	0.2 mg/ml (after reconstitution)
Purification	Affinity Chromatography
Conjugation	Unconjugated
Storage Condition	Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.
Gene Name	CD14 molecule
Database Link	Entrez Gene 929 Human UniProt ID P08571
Background	CD14 is a 53'000 - 55'000 Mr glycoprotein strongly expressed on the surface of monocytes and weakly on the surface of granulocytes. It is anchored to the surface by linkage to glycosyl phosphatidylinositol (GPI). CD14 functions as a receptor for endotoxin and is the major receptor involved in the lethal response to both endotoxin and Gram-negative bacteria. In addition to membrane CD14, soluble forms of CD14 can be detected in serum and tissue culture supernatants of cells transfected with CD14.
Synonyms	CD14
Reference Data

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