CD18 (ITGB2) (activation epitope) Mouse Monoclonal Antibody [Clone ID: MEM-148]

Specifications

Product Data
Clone Name	MEM-148
Applications	FC, FN, IP, WB
Recommend Dilution	Flow cytometry: Recommended dilution: 1-5 μg/ml. The antibody MEM-148 is an excellent marker of activated myeloid cells. Western blotting: Non-reducing conditions.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Peripheral blood mononuclear cells
Specificity	The antibody recognizes the epitope exposed on free human CD18 chains unassociated with CD11 as well as on high-affinity state of LFA-1. CD18 (integrin b2 subunit; b2 integrin) is a 90-95 kDa type I transmembrane protein expressed on all leukocytes.
Formulation	PBS, pH 7.4, with 15 mM Sodium Azide as preservative State: Purified State: Liquid purified Ig fraction (> 95% pure by PAGE)
Concentration	lot specific
Purification	Protein A Affinity Chromatography
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C. DO NOT FREEZE!
Gene Name	integrin subunit beta 2
Database Link	Entrez Gene 3689 Human UniProt ID P05107
Background	CD18, integrin b2 subunit, forms heterodimers with four types of CD11 molecule to constitute leukocyte (b2) integrins: aLb2 (CD11a/CD18, LFA-1), aMb2 (CD11b/CD18, Mac-1, CR3), aXb2 (CD11c/CD18) and aDb2 (CD11d/CD18). In most cases, the response mediated by the integrin is a composite of the functions of its individual subunits. These integrins are essential for proper leukocyte migration, mediating intercellular contacts. Absence of CD18 leads to leukocyte adhesion deficiency-1; severe reduction of CD18 expression leads to the development of a psoriasiform skin disease. CD18 is also a target of Mannheimia (Pasteurella) haemolytica leukotoxin and is sufficient to mediate leukotoxin-mediated cytolysis.
Synonyms	Integrin beta-2, MFI7
Reference Data

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