CD18 (ITGB2) (activation epitope) Mouse Monoclonal Antibody [Clone ID: MEM-148]

Specifications

Product Data
Clone Name	MEM-148
Applications	FC, IHC, IP, WB
Recommend Dilution	Suitable for Immunohistochemistry on frozen sections ( 0.2 µg/ml, 1/2000), Flow cytometry (use 5 µl of the suggested working dilution to label 10e6 cells), Immunoprecipitation and Western blotting (non-reduced). Suggested positive control: Human tonsil.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Specificity	MEM-148 recognizes the β2 integrin subunit that pairs with each of four distinctive CD11 α subunits to form the CD11/CD18 integrin heterodimers. MEM-148 potently promoted adhesion of LFA-1/K562 transfectants and neutrophils to their respective immobilized ligands, suggesting it to be one of the select class of monoclonal antibodies which can act on the ectodomain of the integrin to induce adhesion. Expression of the epitope recognized by MEM-148 is minimal on β2 integrin transfectants, T cells, eosinophils, monocytes and neutrophils.
Formulation	PBS, pH 7.2, containing 10 mg/ml bovine serum albumin (BSA) as a stabilizer and 0.05% sodium azide as a preservative. State: Purified State: Lyophilized diluted ascites.
Concentration	0.4 mg/ml
Conjugation	Unconjugated
Storage Condition	Store the antibody at 2-8°C for one month or (in aliquots) at -20°C for longer. Do not freeze working dilutions Avoid repeated freezing and thawing.
Gene Name	integrin subunit beta 2
Database Link	Entrez Gene 3689 Human UniProt ID P05107
Background	CD18 is an integrin. Integrins are integral cell surface proteins composed of an alpha chain and a beta chain. A given chain may combine with multiple partners resulting in different integrins. For example, beta 2 combines with the alpha L chain to form the integrin LFA1, and combines with the alpha M chain to form the integrin Mac1. Integrins are known to participate in cell adhesion as well as cell surface mediated signalling. CD18 is expressed by most leucocytes. Defects in the CD18 gene result in leukocyte adhesion deficiency.
Synonyms	Integrin beta-2, MFI7
Note	Protocol: Protocol with frozen, ice-cold acetone-fixed sections: The whole procedure is performed at room temperature 1. Wash in PBS 2. Block endogenous peroxidase 3. Wash in PBS 4. Block with 10% normal goat serum in PBS for 30min. in a humid chamber 5. Incubate with primary antibody (dilution see datasheet) for 1h in a humid chamber 6. Wash in PBS 7. Incubate with secondary antibody (peroxidase-conjugated goat anti mouse IgG+IgM (H+L) minimal-cross reaction to human) for 1h in a humid chamber 8. Wash in PBS 9. Incubate with AEC substrate (3-amino-9-ethylcarbazol) for 12min. 10. Wash in PBS 11. Counterstain with Mayer's hemalum.
Reference Data

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