Cd68 Mouse Monoclonal Antibody [Clone ID: ED1]

Specifications

Product Data
Clone Name	ED1
Applications	FC, IHC
Recommend Dilution	Flow Cytometry: Use 10 µl of neat antibody to label 10e6 cells in 100 µl. (Results maybe enhanced using membrane permeabilisation). Immunohistochemistry on Frozen sections: 1/10.
Reactivity	Bovine, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Rat spleen cells
Specificity	CD68 antibody (Cat.-No BM4000B) recognizes a single chain glycoprotein of 110kDa that is expressed predominantly on the lysomal membrane of myeloid cells. Weak cell surface expression also occurs. The antigen is expressed by the majority of tissue macrophages, and weakly be peripheral blood granulocytes. Studies have shown that the antigen recognised by ED1 is the rat homologue of Human CD68. Negative Species: Horse.
Formulation	PBS, pH 7.2 Label: Biotin State: Liquid purified IgG fraction Stabilizer: 1% BSA Preservative: 0.09% Sodium Azide
Concentration	lot specific
Purification	Affinity Chromatography on Protein A
Conjugation	Biotin
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	Cd68 molecule
Database Link	Entrez Gene 287435 Rat UniProt ID Q4FZY1
Background	The CD68 antigen is a 37kD transmembrane protein that is post-translationally glycosylated to give a protein of 87-115kD. CD68 is specifically expressed by tissue macrophages, Langerhans cells and at low levels by dendritic cells. It could play a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. It binds to tissue- and organ-specific lectins or selectins, allowing homing of macrophage subsets to particular sites. Rapid recirculation of CD68 from endosomes and lysosomes to the plasma membrane may allow macrophages to crawl over selectin bearing substrates or other cells.
Synonyms	Gp110, Macrosialin, Macrophage marker
Reference Data

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